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1. (WO1999037795) PROMOTER AND REGULATOR SEQUENCES $i(Ha ds10 G1): A GENE LEA OF SUNFLOWER EXPRESSED EXCLUSIVELY IN SEEDS FROM THE MATURATION PHASE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/1999/037795 International Application No.: PCT/ES1999/000017
Publication Date: 29.07.1999 International Filing Date: 23.01.1999
Chapter 2 Demand Filed: 19.08.1999
IPC:
C07K 14/415 (2006.01) ,C12N 15/82 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415
from plants
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
Applicants:
PRIETO-DAPENA, Maria Pilar [ES/ES]; ES (UsOnly)
ALMOGUERA ANTOLINEZ, María Concepción [ES/ES]; ES (UsOnly)
JORDANO FRAGA, Juan Bautista [ES/ES]; ES (UsOnly)
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS [ES/ES]; Calle Serrano, 117 E-28006 Madrid, ES (AllExceptUS)
Inventors:
PRIETO-DAPENA, Maria Pilar; ES
ALMOGUERA ANTOLINEZ, María Concepción; ES
JORDANO FRAGA, Juan Bautista; ES
Agent:
UNGRÍA LÓPEZ, Javier; Avda. Ramón y Cajal, 78 E-28043 Madrid, ES
Priority Data:
P 980012223.01.1998ES
Title (EN) PROMOTER AND REGULATOR SEQUENCES $i(Ha ds10 G1): A GENE LEA OF SUNFLOWER EXPRESSED EXCLUSIVELY IN SEEDS FROM THE MATURATION PHASE
(FR) PROMOTEUR ET SEQUENCES REGULATRICES DE $i(Ha ds10 G1) GENE LEA DE TOURNESOL EXPRIME EXCLUSIVEMENT DANS LES GRAINES DEPUIS LA PHASE DE MATURATION
(ES) PROMOTOR Y SECUENCIAS REGULADORAS DE $i(Ha ds10 G1): UN GEN LEA DE GIRASOL EXPRESADO EXCLUSIVAMENTE EN SEMILLAS DESDE LA FASE DE MADURACION
Abstract:
(EN) The present invention discloses the isolation and characterization in transgenic tobacco plants of the promoter and regulator sequences of a gene LEA-I of sunflower, $i(Ha ds10 G1). These sequences present characteristics which are extremely appropriate to be used in the modification of seeds (for example of reserve substances). The advantages of their possible use in transgenic plants are demonstrated through examples such as studies related to the accumulation and location of RNAm Ha ds10 in the homologous system. Said studies show both the high expression levels reached during embryogenesis from the early maturation phases and the absolute specificity of the seed, together with a homogenous location in embryos which is finally restricted essentially to the soft tissue in palisade of the cotyledons, a tissue specialized in the accumulation of reserve substances in the sunflower.
(FR) L'invention consiste à isoler et caractériser chez le plantes transgéniques de tabac le promoteur et les séquences régulatrices d'un gène LEA-I de tournesol, $i(Ha) $i(ds10 G1). Ces séquences présentent des caractéristiques se prêtant parfaitement à leur utilisation dans la modification de graines (p. ex. de substances de restes). Les avantages, découlant de son utilisation sur les plantes transgéniques, ont été démontrés par le biais d'exemples, tels que des études sur l'accumulation et la localisation de l'ARNm Ha ds10 dans le système homologue. Ces études mettent en avant les niveaux élevés d'expression du gène atteints au cours de l'embryogenèse depuis les phases précoces de la maturation et sa spécificité absolue par rapport à la graine, ainsi que sa localisation homogène de l'ARNm chez les embryons qui se limite essentiellement au parenchyme en palissade des cotylédons, tissus spécialisés dans l'accumulation de substances de réserves dans le tournesol.
(ES) Con la presente invención aislamos y caracterizamos en plantas transgénicas de tabaco, el promotor y las secuencias reguladoras de un gen LEA-I de girasol, $i(Ha ds10) $i(G1). Estas secuencias presentan unas características muy apropiadas para su uso en la modificación de semillas (por ej. de sustancias de reservas). Las ventajas de su posible uso en plantas transgénicas se muestran mediante ejemplos como estudios de la acumulación y localización del ARNm Ha ds10 en el sistema homólogo. Estos estudios muestran tanto los elevados niveles de expresión alcanzados durante la embriogénesis desde fases tempranas de la maduración, como sus absoluta especificidad de semilla, acompañada de una localización homogénea en embriones que acaba restringiéndose fundamentalmente al parénquima en empalizada de los cotiledones, un tejido especializado en la acumulación de sustancias de reservas en el girasol.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, UA, UG, US, UZ, VN, YU, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, SD, SZ, UG, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Spanish (ES)
Filing Language: Spanish (ES)