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1. (WO1999013065) NOVEL PRIMER AND USE THEREOF

Pub. No.:    WO/1999/013065    International Application No.:    PCT/JP1998/004076
Publication Date: Mar 18, 1999 International Filing Date: Sep 10, 1998
IPC: C07K 14/47
C12Q 1/68
Applicants: SNOW BRAND MILK PRODUCTS CO., LTD.

IMAMURA, Mio

ITAGAKI, Yasuharu

TANIMOTO, Morimasa

Inventors: IMAMURA, Mio

ITAGAKI, Yasuharu

TANIMOTO, Morimasa

Title: NOVEL PRIMER AND USE THEREOF
Abstract:
A novel primer to be used in the reverse transcription polymerase chain reaction in order to amplify αs1-casein gene and keratin gene contained in milk and calculate the expression ratio of these genes. By using this ratio as an indication, the pathological conditions of mammalian mastitis are examined or the milk protein synthesis level in mammary gland cells is measured. Thus, the examination/measurement can be conveniently, quickly and accurately effected without excising the mammary gland tissue.