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1. (WO1999009150) METHOD OF INTRODUCING MODIFICATIONS INTO A GENE

Pub. No.:    WO/1999/009150    International Application No.:    PCT/US1997/014507
Publication Date: Fri Feb 26 00:59:59 CET 1999 International Filing Date: Tue Aug 19 01:59:59 CEST 1997
IPC: C07K 14/47
C12N 15/85
Applicants: BAYER CORPORATION
Inventors: WIRAK, Dana, Owen
Title: METHOD OF INTRODUCING MODIFICATIONS INTO A GENE
Abstract:
The present invention relates to a gene targeting vector and a method of using it to modify nucleic acid sequences. A gene targeting vector in accordance with the invention can comprise: a nucleotide sequence which is effective to achieve homologous recombination at a predefined position of a target gene, operably linked to the 5' terminus of a nucleotide coding sequence which, when inserted into a target gene, codes for at least one amino acid whose identity and/or position is not naturally-occurring in the target gene, and a nucleotide sequence which is effective to achieve homologous recombination at a predefined position of the target gene, operably linked to the 3' terminus of said nucleotide coding sequence. The nucleotide coding sequence can code without interruption for an amino acid sequence, where the amino acid sequence is coded for by two or more exons in a naturally-occurring gene.