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1. (WO1999009062) CELL CHEMOTACTIC FACTOR (CCTF) ORIGINATING IN MAMMALIAN TOOTH PRECEMENT OR CEMENT, METHOD FOR PURIFYING THE SAME, AND NOVEL CONNECTIVE TISSUE ADHESION PROMOTERS CONTAINING THE SAME AS THE ACTIVE INGREDIENT

Pub. No.:    WO/1999/009062    International Application No.:    PCT/JP1998/003619
Publication Date: Fri Feb 26 00:59:59 CET 1999 International Filing Date: Fri Aug 14 01:59:59 CEST 1998
IPC: A61K 38/00
C07K 14/52
Applicants: KANEBO LIMITED

MUNEKATA, Masanobu

NISHIMURA, Kazuaki

Inventors: MUNEKATA, Masanobu

NISHIMURA, Kazuaki

Title: CELL CHEMOTACTIC FACTOR (CCTF) ORIGINATING IN MAMMALIAN TOOTH PRECEMENT OR CEMENT, METHOD FOR PURIFYING THE SAME, AND NOVEL CONNECTIVE TISSUE ADHESION PROMOTERS CONTAINING THE SAME AS THE ACTIVE INGREDIENT
Abstract:
A cell chemotactic factor (CCTF) originating in mammalian tooth precement or cement which has a molecular weight of 67,000 ± 1,000 determined by SDS-PAGE. A method which comprises taking up the precement or cement from extracted mammalian teeth, immersing the same in physiological saline optionally containing collagenase, and purifying the above CCTF from the resultant eluate by molecular weight fractionation, ion exchange adsorption chromatography and hydroxy-apatite adsorption chromatography.