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1. WO1999008513 - CRYOPROTECTANT REMOVAL METHOD AND APPARATUS

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Publication Number WO/1999/008513
Publication Date 25.02.1999
International Application No. PCT/US1998/017105
International Filing Date 18.08.1998
Chapter 2 Demand Filed 19.03.1999
IPC
A01N 1/02 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
CPC
A01N 1/0205
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
0205Chemical aspects
A01N 1/0221
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
0205Chemical aspects
021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
0221Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
A01N 1/0242
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
0236Mechanical aspects
0242Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
A01N 1/0268
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
1Preservation of bodies of humans or animals, or parts thereof
02Preservation of living parts
0236Mechanical aspects
0263Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
0268Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
Applicants
  • BIOPORE, INC. [US]/[US]
Inventors
  • AMANN, Rupert, P.
  • HAMMERSTEDT, Roy, H.
Agents
  • ORKIN, Russell, D.
Priority Data
60/056,60720.08.1997US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) CRYOPROTECTANT REMOVAL METHOD AND APPARATUS
(FR) PROCEDE ET APPAREIL D'EXTRACTION D'UN CRYOPROTECTEUR
Abstract
(EN) A process and apparatus in which cryoprotectant in cryopreserved samples is removed with the use of turbulence and convective-dispersion around the cells within the primary cryopreservation container are described. Convective-dispersion is applied via the combined action of gravity and pulsative transmembrane flow of buffer through the pores of containers fabricated from appropriate membrane material. Use of mechanical forces to aid the process of cryoprotectant removal permits the benefits of slow cryoprotectant removal to be retained, while the actual removal time is decreased.
(FR) L'invention concerne un procédé et un appareil permettant d'extraire un cryoprotecteur d'échantillons cryoconservés. Selon ledit procédé, on extrait le cryoprotecteur en utilisant la turbulence et la dispersion convective se produisant autour des cellules, à l'intérieur du récipient primaire de cryoconservation. La dispersion convective est produite par l'action combinée de la gravité et de l'écoulement pulsatoire transmembranaire de tampon à travers les pores de récipients fabriqués à partir d'un matériau pour membranes approprié. L'utilisation de forces mécaniques pour assister le processus de l'extraction du cryoprotecteur permet de conserver les avantages d'une extraction lente de cryoprotecteur tout en ayant un temps d'extraction réel nettement réduit.
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