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1. WO1999005255 - METHODS FOR ENHANCING OR REDUCING PREIMPLANTATION EMBRYO SURVIVAL RATES

Publication Number WO/1999/005255
Publication Date 04.02.1999
International Application No. PCT/US1998/015124
International Filing Date 22.07.1998
Chapter 2 Demand Filed 23.02.1999
IPC
A61K 38/00 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C07K 14/47 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
C07K 14/74 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
74Major histocompatibility complex (MHC)
CPC
A61K 38/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C07K 14/4747
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
4701not used
4747Apoptosis related proteins
C07K 14/70539
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
70503Immunoglobulin superfamily
70539MHC-molecules, e.g. HLA-molecules
C12N 15/8775
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
873Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
877Techniques for producing new mammalian cloned embryos
8775Murine embryos
C12N 5/0006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0006Modification of the membrane of cells, e.g. cell decoration
C12N 5/0604
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0603Embryonic cells
0604Whole embryos; Culture medium therefor
Applicants
  • NORTHEASTERN UNIVERSITY [US]/[US] (AT, AU, BE, CA, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IL, IT, JP, LU, MC, NL, PT, SE)
  • WARNER, Carol, M. [US]/[US] (UsOnly)
  • McELHINNY, Abigail, S. [US]/[US] (UsOnly)
Inventors
  • WARNER, Carol, M.
  • McELHINNY, Abigail, S.
Agents
  • SCHURGIN, Stanley, M.
Priority Data
60/053,49923.07.1997US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR ENHANCING OR REDUCING PREIMPLANTATION EMBRYO SURVIVAL RATES
(FR) METHODES D'AUGMENTATION OU DE REDUCTION DES TAUX DE SURVIE D'EMBRYONS DE PREIMPLANTATION
Abstract
(EN) The present invention relates to the use of 'protein painting' (the addition of exogenous, lipid-modified proteins) to improve embryonic viability, development, and implantation. It is shown here that a lipid-modified protein, e.g., GPI-modified exogenous Qa-2 protein, spontaneously incorporates into the plasma membrane of B6.K1 T cells and onto $i(Ped slow) preimplantation embryos. It is also shown that, given enough time after incorporation, the exogenous Qa-2 protein increases the rate of development of early embryos cultured $i(in vitro), most likely by diffusing in the plasma membrane until it is 'connected' to its functional signaling machinery. Protein painting of embryos has the potential, therefore, to be a valuable tool in enhancing embryo development and implantation.
(FR) La présente invention concerne l'utilisation d'une 'peinture de protéines' (l'addition de protéines exogènes modifiées par lipides) pour améliorer la viabilité, le développement et l'implantation d'embryons. On a démontré ici qu'une protéine modifiée par un lipide, par exemple, une protéine Qa-2 exogène modifiée par GPI (glycosylephosphatydilinositol ajoutée de manière post-translationnelle), s'intègre spontanément à la membrane du plasma de lymphocytes T B6.K1 et sur des embryons de préimplantation $i(Ped lent) (développement d'embryons de préimplantation lent). On a également démontré que, lorsque suffisamment de temps s'est écoulé après l'incorporation, la protéine Qa-2 exogène augmente le taux de développement d'embryons précoces mis en culture $i(in vitro), le plus probablement par diffusion dans la membrane plasmique jusqu'à ce qu'elle soit 'connectée' à son mécanisme de signalisation fonctionnelle. La peinture protéique d'embryons présente par conséquent la caractéristique potentielle d'être un outil de valeur dans l'amélioration du développement et de l'implantation d'embryons.
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