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1. (WO1999004820) CYTOLYSIS OF TARGET CELLS BY SUPERANTIGEN CONJUGATES INDUCING T-CELL ACTIVATION

Pub. No.:    WO/1999/004820    International Application No.:    PCT/EP1998/004219
Publication Date: Feb 4, 1999 International Filing Date: Jul 2, 1998
IPC: A61K 47/48
C07K 14/47
Applicants: PHARMACIA & UPJOHN AB
SOEGAARD, Morten
ABRAHMSEN, Lars
LANDO, Peter
FORSBERG, Göran
KALLAND, Terje
DOHLSTEN, Mikael
Inventors: SOEGAARD, Morten
ABRAHMSEN, Lars
LANDO, Peter
FORSBERG, Göran
KALLAND, Terje
DOHLSTEN, Mikael
Title: CYTOLYSIS OF TARGET CELLS BY SUPERANTIGEN CONJUGATES INDUCING T-CELL ACTIVATION
Abstract:
A method for inactivating target cells in the presence of T cells by bringing the two types of cells in contact with a superantigen (SAG) in the presence of an immune modulator, characterized in that at least one of the superantigen and the immune modulator is in the form of a conjugate between a 'free' superantigen (Sag) and a moiety targeting the conjugate to the target cells. A superantigen conjugate complying with the formula (I): (T)x(Sag)y(IM)z; a) T is a targeting moiety, Sag corresponds to a free superantigen, IM is an immune modulator that is not a superantigen and T, Sag and IM are linked together via organic linkers B; b) x, y and z are integers that typically are selected among 0-10 and represent the number of moieties T, Sag and IM, respetively, in a given conjugate molecule, with the provision that y > 0 and also one or both of x and z > 0. The superantigen conjugate is preferably a triple fusion protein. A targeted immune modulator, characterized in that it is a conjugate between a targeting moiety (T''') and a modified immune modulator (IM'''). The conjugate complies with a formula analogous to formula (I) except for the imperative presence of the modified immune modulator. A superantigen moiety may be present. A DNA molecule encoding a superantigen and an immune modulator.