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1. WO1999003988 - PROCESS FOR PRODUCING PURINE NUCLEOSIDES VIA FERMENTATION

Publication Number WO/1999/003988
Publication Date 28.01.1999
International Application No. PCT/JP1998/003239
International Filing Date 17.07.1998
Chapter 2 Demand Filed 25.12.1998
IPC
C12P 19/40 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
38Nucleosides
40having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
CPC
C12N 1/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
20Bacteria
C12N 1/205
C12N 9/1077
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
1048Glycosyltransferases (2.4)
1077Pentosyltransferases (2.4.2)
C12P 19/38
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
38Nucleosides
C12P 19/40
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
38Nucleosides
40having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
C12R 2001/19
Applicants
  • AJINOMOTO CO., INC. [JP]/[JP] (AT, BE, BR, CH, CN, CY, DE, DK, ES, FI, FR, GB, GR, ID, IE, IT, JP, KR, LU, MC, NL, PT, SE)
  • MATSUI, Hiroshi [JP]/[JP] (UsOnly)
  • KAWASAKI, Hisashi [JP]/[JP] (UsOnly)
  • SHIMAOKA, Megumi [JP]/[JP] (UsOnly)
  • TAKENAKA, Yasuhiro [JP]/[JP] (UsOnly)
  • KURAHASHI, Osamu [JP]/[JP] (UsOnly)
Inventors
  • MATSUI, Hiroshi
  • KAWASAKI, Hisashi
  • SHIMAOKA, Megumi
  • TAKENAKA, Yasuhiro
  • KURAHASHI, Osamu
Agents
  • TOYAMA, Tsutomu
Priority Data
9/19460318.07.1997JP
Publication Language Japanese (ja)
Filing Language Japanese (JA)
Designated States
Title
(EN) PROCESS FOR PRODUCING PURINE NUCLEOSIDES VIA FERMENTATION
(FR) PROCEDE DE PRODUCTION DE NUCLEOSIDES DE PURINE PAR FERMENTATION
Abstract
(EN) A process for producing purine nucleosides which comprises breeding and culturing microorganisms having a gene encoding an enzyme wherein the feedback inhibition has been substantially relieved by substituting one or two amino acid residues in PRPP amidotransferase encoded by $i(Escherichia coli) purF, a gene encoding a protein wherein the repressor in the purine nucleotide biosynthesis system encoded by purR has been inactivated and a gene encoding an enzyme wherein purine nucleoside phosphorylase encoded by deoD has been inactivated, a gene encoding an enzyme wherein succinyl-AMP synthase encoded by purA has been inactivated, a gene encoding an enzyme wherein 6-phosphogluconate dehydratase encoded by edd has been inactivated and a gene encoding an enzyme wherein phosphoglucose isomerase encoded by pgi has been inactivated, or the like.
(FR) L'invention concerne un procédé de production de nucléosides de purine, le procédé consistant à sélectionner et à cultiver des micro-organismes possédant un gène codant une enzyme dans laquelle la rétro-inhibition a été sensiblement améliorée par substitution d'un ou de deux résidus d'acides aminés dans PRPP amidotransférase codée par $i(Escherichia coli) purF, un gène codant une protéine dans laquelle le répresseur dans le système de biosynthèse nucléotidique de purine codé par purR est inactivé et un gène codant une enzyme dans laquelle un nucléoside de purine phosphorylase codée par deoD est inactivé, un gène codant une enzyme dans laquelle une succinyl-AMP synthase codée par purA est inactivée, un gène codant une enzyme dans laquelle 6-phosphogluconate déshydratase codée par edd est inactivée et un gène codant une enzyme dans laquelle une phosphoglucose isomérase codée par pgi est inactivée, ou analogues.
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