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1. (WO1998053308) ON-LINE LIQUID SAMPLE DEPOSITION INTERFACE FOR MATRIX ASSISTED LASER DESORPTION IONIZATION-TIME OF FLIGHT (MALDI-TOF) MASS SPECTROSCOPY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/053308 International Application No.: PCT/US1998/010537
Publication Date: 26.11.1998 International Filing Date: 22.05.1998
Chapter 2 Demand Filed: 23.12.1998
IPC:
G01N 1/00 (2006.01) ,G01N 1/22 (2006.01) ,H01J 49/04 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
02
Devices for withdrawing samples
22
in the gaseous state
H ELECTRICITY
01
BASIC ELECTRIC ELEMENTS
J
ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
49
Particle spectrometers or separator tubes
02
Details
04
Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
Applicants:
NORTHEASTERN UNIVERSITY [US/US]; 360 Huntington Avenue Boston, MA 02115, US
Inventors:
KARGER, Barry, L.; US
FORET, Frantisek; US
PREISLER, Jan; US
Agent:
HEINE, Holliday, C. ; Weingarten, Schurgin, Gagnebin & Hayes LLP Ten Post Office Square Boston, MA 02109, US
Priority Data:
60/047,48923.05.1997US
Title (EN) ON-LINE LIQUID SAMPLE DEPOSITION INTERFACE FOR MATRIX ASSISTED LASER DESORPTION IONIZATION-TIME OF FLIGHT (MALDI-TOF) MASS SPECTROSCOPY
(FR) INTERFACE DE DEPOT EN LIGNE D'ECHANTILLONS LIQUIDES POUR SPECTROSCOPIE DE MASSE A TEMPS DE VOL A IONISATION-DESORPTION LASER ASSISTEE PAR MATRICE (MALDI-TOF)
Abstract:
(EN) An universal interface for continuous on-line liquid sample introduction directly into the source chamber (5) of a time-of-flight mass spectrometer (20) for matrix-assisted-laser-desorption-ionization (MALDI) analysis is disclosed. The liquid sample includes a liquid or solid matrix material used in conventional MALDI analysis and is deposited directly onto a moving sample holder (22) in the source chamber (5). The sample holder (22) is at subatmospheric pressure, causing the rapid drying of the sample. The dried sample is subsequently desorbed from the sample holder (22) by a nitrogen laser (28) in a desorption region (26). The method of the invention is particularly amenable to multiplexing, the parallel deposition of multiple samples from an array of capillaries or microchip channels (24), with the subsequent sequential desorption by a scanning laser. This format is particularly useful for high throughput mass spectrometric analysis.
(FR) L'invention concerne une interface universelle permettant d'introduire en ligne et en continu des échantillons liquides directement dans la chambre source (5) d'un spectromètre (20) de masse à temps de vol en vue d'une analyse à ionisation-désorption laser assistée par matrice (MALDI). L'échantillon liquide renferme une matrice solide ou liquide que l'on utilise lors des analyses MALDI classiques et il est déposé directement sur un porte-échantillons (22) mobile dans la chambre source (5). Le porte-échantillons (22) est à pression subatmosphérique, d'où un séchage rapide de l'échantillon. L'échantillon séché est ensuite désorbé du porte-échantillons (22) au moyen d'un laser à azote (28) dans une zone de désorption (26). Le procédé de l'invention permet un travail sur plusieurs échantillons, à savoir le dépôt parallèle de divers échantillons à partir d'un ensemble de capillaires ou de canaux micropuce (24), la désorption séquentielle qui s'ensuit étant assurée par un laser de lecture. Cet arrangement est particulièrement utile pour des analyses de spectrométrie de masse à haut rendement.
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Designated States: CA, JP
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0986746JP2002502543