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1. (WO1998053056) METHOD FOR PRODUCING LONG DNA CONSTRUCTS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/053056 International Application No.: PCT/DE1998/001421
Publication Date: 26.11.1998 International Filing Date: 18.05.1998
Chapter 2 Demand Filed: 16.12.1998
IPC:
C12N 15/10 (2006.01) ,C12N 15/74 (2006.01) ,C12N 15/85 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
Applicants:
SCHINDELHAUER, Dirk [DE/DE]; DE
COOKE, Howard, J. [GB/GB]; GB (UsOnly)
Inventors:
SCHINDELHAUER, Dirk; DE
COOKE, Howard, J.; GB
Agent:
HUBER, Bernard; Truderinger Strasse 246 D-81825 München, DE
Priority Data:
197 20 839.817.05.1997DE
Title (DE) HERSTELLUNGSVERFAHREN FÜR LANGE DNA-KONSTRUKTE
(EN) METHOD FOR PRODUCING LONG DNA CONSTRUCTS
(FR) PROCEDE DE PREPARATION DE PRODUITS DE SYNTHESE D'ADN LONGS
Abstract:
(DE) Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung eines DNA-Konstruktes, umfassend die Kombination zweier oder mehrerer DNAs mittels Rekombination in geschmolzener Agarose. Ferner betrifft die Erfindung hierfür verwendbare Vektoren sowie ein Verfahren zur Bereitstellung großer DNAs, insbesondere BACs oder PACs.
(EN) The invention relates to a method for producing a DNA construct, whereby two or several DNAs are recombined in melted agarose. The invention also relates to vectors which can be used therefor, in addition to a method for providing large DNAs, specially BACs or PACs.
(FR) L'invention concerne un procédé pour la préparation d'un produit de synthèse d'ADN, comportant l'association d'au moins deux ADN par recombinaison dans l'agarose fondu. L'invention concerne d'autre part des vecteurs utilisables à cet effet, ainsi qu'un procédé pour la préparation d'ADN longs, notamment des chromosomes artificiels bactériens ou des chromosomes artificiels du phage P1.
Designated States: JP, US
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: German (DE)
Filing Language: German (DE)
Also published as:
US6331397