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1. (WO1998052018) IDENTIFICATION OF OBJECTS BY MEANS OF MULTIPLE IMAGING

Pub. No.:    WO/1998/052018    International Application No.:    PCT/US1998/009689
Publication Date: Fri Nov 20 00:59:59 CET 1998 International Filing Date: Wed May 13 01:59:59 CEST 1998
IPC: G01N 15/00
G01N 15/10
G01N 15/14
Applicants: APPLIED IMAGING CORPORATION
RAVKIN, Ilya
Inventors: RAVKIN, Ilya
Title: IDENTIFICATION OF OBJECTS BY MEANS OF MULTIPLE IMAGING
Abstract:
Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multi-bandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglubin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity. The brightfield and fluorescence images may be sequentially acquired or derived from a single image where the UV excitation and visible illumination occur simultaneously.