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1. (WO1998051818) METHOD FOR TREATMENT OF DEOXYRIBONUCLEIC ACID SEQUENCE BY RESTRICTASE
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/051818 International Application No.: PCT/UA1997/000004
Publication Date: 19.11.1998 International Filing Date: 14.05.1997
IPC:
A61K 41/00 (2006.01) ,C12N 15/10 (2006.01)
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
41
Medicinal preparations obtained by treating materials with wave energy or particle radiation
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
KHRAPUNOV, Volodymyr Mykolaiovych [UA/UA]; UA
KHRAPUNOV, Sergy Mykolaiovytch [UA/UA]; UA
Inventors:
KHRAPUNOV, Volodymyr Mykolaiovych; UA
KHRAPUNOV, Sergy Mykolaiovytch; UA
Agent:
INSTITUTE OF CARDIO-VASCULAR SURGERY; Patent Dept. Protasiv Yar, 11 Kiev, 252601, UA
Priority Data:
Title (EN) METHOD FOR TREATMENT OF DEOXYRIBONUCLEIC ACID SEQUENCE BY RESTRICTASE
(FR) PROCEDE DE TRAITEMENT DE SEQUENCE D'ACIDE DESOXYRIBONUCLEIQUE (ADN) PAR RESTRICTASE
Abstract:
(EN) The present invention relates to chemistry and, more specifically, to gene engineering. Method for treatment of deoxyribonucleic acid sequence by restrictase, suitable for said treatment of practically any of said DNA sequence, comprises the following steps: binding co-valently a chemical compound capable to recognize specifically a concrete DNA site (component A) with a photoligand (component B) to obtain a new substance; adding the substance obtained to said DNA sequence to receive a complex; and illuminating by light the complex received with wave lengths selected from spectrum covered visible and ultraviolet ranges. The invention may be utilized for DNA site-specific restriction and site-specific mutagenesis in gene engineering, biotechnology, medicine, veterinary.
(FR) L'invention concerne la chimie et, plus précisément, le génie génétique. On décrit un procédé de traitement de séquence d'ADN par restrictase, qui convient pour ledit traitement par restrictase appliqué à toute séquence d'ADN, et qui comprend les étapes suivantes : établissement d'une liaison covalente entre un composé chimique capable de reconnaître spécifiquement un site d'ADN concret (élément A) et un photoligand (élément B), pour obtenir une nouvelle substance; adjonction de la substance résultante à la séquence d'ADN considérée, pour la réception d'un complexe; et exposition à un rayonnement lumineux du complexe reçu, à des longueurs d'ondes choisies dans les gammes d'ondes couvertes par le spectre visible et le spectre des rayons ultraviolets. On peut utiliser l'invention pour la restriction propre aux sites de l'ADN et la mutagénèse propre aux sites, en génie génétique et en biotechnologie ainsi que dans les applications médicales et vétérinaires.
Designated States: AL, AM, AU, BB, BG, BR, CA, CN, CZ, EE, FI, GE, HU, IS, JP, KG, KP, KR, LK, LR, LT, LV, MD, MG, MK, MN, MX, NO, NZ, PL, RO, SG, SI, SK, TR, TT, UA, US, UZ, VN
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU1998052377