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1. (WO1998051808) METHODS FOR PRODUCING RETROVIRAL VECTORS WITH AN ALTERED TROPISM
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/051808 International Application No.: PCT/NL1998/000260
Publication Date: 19.11.1998 International Filing Date: 12.05.1998
Chapter 2 Demand Filed: 09.12.1998
IPC:
C07K 14/16 (2006.01) ,C12N 15/867 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005
from viruses
08
RNA viruses
15
Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
155
Lentiviridae, e.g. human immunodeficiency virus (HIV), visna-maedi virus, equine infectious anaemia virus
16
HIV-1
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
86
Viral vectors
867
Retroviral vectors
Applicants:
ACADEMISCH MEDISCH CENTRUM AMSTERDAM [NL/NL]; Meibergdreef 9 NL-1105 AZ Amsterdam, NL (AllExceptUS)
BERKHOUT, Benjamin [NL/NL]; NL (UsOnly)
DAS, Atze, Taede [NL/NL]; NL (UsOnly)
Inventors:
BERKHOUT, Benjamin; NL
DAS, Atze, Taede; NL
Agent:
SMULDERS, Th., A., H., J.; Vereenigde Octrooibureaux Nieuwe Parklaan 97 NL-2587 BN The Hague, NL
OTTEVANGERS, S., U.; Vereenigde Octrooibureaux Nieuwe Parklaan 97 NL-2587 BN The Hague, NL
Priority Data:
97201422.912.05.1997EP
Title (EN) METHODS FOR PRODUCING RETROVIRAL VECTORS WITH AN ALTERED TROPISM
(FR) PROCEDES DE PRODUCTION DE VECTEURS RETROVIRAUX PRESENTANT UN TROPISME MODIFIE
Abstract:
(EN) The invention relates to the field of molecular biology and virology. It particularly relates to the field of genetic engineering techniques and the use of these techniques in preparing novel pharmaceuticals, in particular in the field of gene therapy and to methods for producing novel retroviral vectors, these vectors and recombinant viruses obtainable by the methods. The invention provides a method for producing a retrovirus or a recombinant virus derived therefrom having an altered tropism, whereby the genome of the virus is modified so that at least one of its translation initiation sites is at least partially functionally inhibited. The invention also provides a method for producing a recombinant vector encoding a recombinant retrovirus having modified tropism, comprising culturing said retrovirus in a host cell to allow for the virus to circumvent an introduced inhibition, identifying the sequence which was mutated to circumvent said inhibition and introducing the mutation in another vector based on a retrovirus or provirus of a retrovirus.
(FR) L'invention porte sur la biologie moléculaire et la virologie. Elle porte en particulier sur des techniques d'ingénieurie génétique et leur mise en oeuvre dans la préparation de nouveaux produits pharmaceutiques utilisés notamment en thérapie génique, ainsi que sur des procédés de production de nouveaux vecteurs rétroviraux, sur lesdits vecteurs et sur des virus recombinants pouvant être obtenus par ces procédés. L'invention porte en outre sur un procédé de production d'un rétrovirus ou virus recombinant dérivé présentant un tropisme modifié, le génome dudit virus étant modifié de sorte qu'au moins un de ses sites promoteurs de translation soit au moins partiellement inhibé de manière fonctionnelle. L'invention porte enfin sur un procédé de production d'un vecteur recombinant codant un rétrovirus de recombinaison présentant un tropisme modifié. Le procédé consiste à cultiver ledit rétrovirus dans une cellule hôte pour permettre au virus de se soustraire à une inhibition introduite, à identifier la séquence mutée pour éluder ladite inhibition, et à introduire la mutation dans un autre vecteur obtenu à partir d'un rétrovirus ou un provirus de ce dernier.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, GH, GM, GW, HU, ID, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, UA, UG, US, UZ, VN, YU, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, SD, SZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0972064JP2001524831 CA2289953AU1998075537