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1. (WO1998051699) METHODS FOR PRODUCTION AND PURIFICATION OF NUCLEIC ACID MOLECULES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/051699 International Application No.: PCT/US1998/009586
Publication Date: 19.11.1998 International Filing Date: 12.05.1998
Chapter 2 Demand Filed: 11.12.1998
IPC:
C12N 15/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
LIFE TECHNOLOGIES, INC. [US/US]; 9800 Medical Center Drive Rockville, MD 20850, US
Inventors:
GRUBER, Christian, E.; US
JESSEE, Joel, A.; US
Agent:
ESMOND, Robert, W. ; Sterne, Kessler, Goldstein & Fox P.L.L.C. Suite 600 1100 New York Avenue, N.W. Washington, DC 20005-3934, US
Priority Data:
60/046,21912.05.1997US
Title (EN) METHODS FOR PRODUCTION AND PURIFICATION OF NUCLEIC ACID MOLECULES
(FR) PROCEDES PERMETTANT DE PRODUIRE ET DE PURIFIER LES MOLECULES D'ACIDE NUCLEIQUE
Abstract:
(EN) The present invention is directed to methods for the production and isolation of nucleic acid molecules. In particular, the invention concerns isolation of mRNA molecules and the production and isolation of nucleic acid molecules (e.g., cDNA molecules or libraries), which may be single- or double-stranded. Additionally, the invention concerns selection and isolation of particular nucleic acid molecules of interest from a sample which may contain a population of molecules. Specifically, the invention concerns affinity-labeled primer-adapter molecules which allow improved isolation and production of such nucleic acid molecules, increasing both product recovery and speed of isolation.
(FR) L'invention concerne des méthodes permettant de produire et d'isoler les molécules d'acide nucléique. Elle concerne notamment l'isolement de molécules d'ARNm, ainsi que la production et l'isolement de molécules d'acide nucléique (par exemple de molécules ou de banques d'ADNc) simple brin ou double brin. Elle concerne en outre la sélection et l'isolement de certaines molécules d'acide nucléique intéressantes à partir d'un échantillon pouvant contenir une population de molécules. Elle concerne spécifiquement des molécules d'adaptateur d'amorce marquées par affinité, qui améliorent l'isolement et la production desdites molécules d'acide nucléique en augmentant à la fois l'extraction du produit et la rapidité de l'isolement.
Designated States: JP
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0981535JP2002502237