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1. (WO1998051698) SYNTHESIS OF OLIGONUCLEOTIDES

Pub. No.:    WO/1998/051698    International Application No.:    PCT/SE1998/000893
Publication Date: Fri Nov 20 00:59:59 CET 1998 International Filing Date: Fri May 15 01:59:59 CEST 1998
IPC: C07H 21/00
Applicants: KWIATKOWSKI, Marek
LANDEGREN, Ulf
NILSSON, Mats
Inventors: KWIATKOWSKI, Marek
LANDEGREN, Ulf
NILSSON, Mats
Title: SYNTHESIS OF OLIGONUCLEOTIDES
Abstract:
A method of preparing an immobilized oligonucleotide having a free 3'-end comprises the steps of: i) preparing an oligonucleotide attached in a first position to a solid support via its 3'-end and having a free 5'-end; ii) binding said oligonucleotide in a second position remote from the 3'-end to the solid support; and iii) selectively releasing the 3'-end of the oligonucleotide from the solid support to obtain the oligonucleotide attached to the support in said second position in a reversed orientation with a free 3'-end.