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1. (WO1998050408) HYBRID RIBOZYMES AND METHODS OF USE

Pub. No.:    WO/1998/050408    International Application No.:    PCT/US1998/009559
Publication Date: Fri Nov 13 00:59:59 CET 1998 International Filing Date: Tue May 12 01:59:59 CEST 1998
IPC: C12N 9/00
C12N 15/11
Applicants: UNIVERSITY OF MASSACHUSETTS
UNIVERSITE DE MONTREAL
Inventors: FOURNIER, Maurille, J.
SAMARSKY, Dmitry, A.
FERBEYRE, Gerardo
CEDERGREN, Robert
Title: HYBRID RIBOZYMES AND METHODS OF USE
Abstract:
New hybrid ribozymes that exhibit the high metabolic stability of small nucleolar ribonucleic acids (snoRNAs), and that cleave target sequences with high efficiencies in either cis (intra-molecular) or trans (inter-molecular) configuration are described. The hybrid ribozymes include (i) a ribozyme catalytic sequence; and (ii) a snoRNA stabilizing motif; wherein the catalytic sequence and the stabilizing motif are arranged to provide the RNA molecule with a three-dimensional configuration in which the catalytic squence is positioned to interact with a target sequence and the stabilizing motif adopts a conformation that stabilizes the RNA molecule, and additionally enables the RNA molecule to localize in the nucleolus of a cell.