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1. (WO1998049537) PHOTOBLEACHABLE LUMINESCENT LAYERS FOR CALIBRATION AND STANDARDIZATION IN OPTICAL MICROSCOPY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/049537 International Application No.: PCT/EP1998/002358
Publication Date: 05.11.1998 International Filing Date: 17.04.1998
Chapter 2 Demand Filed: 02.10.1998
IPC:
G01N 21/64 (2006.01) ,G01N 21/65 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
62
Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63
optically excited
64
Fluorescence; Phosphorescence
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
62
Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63
optically excited
65
Raman scattering
Applicants:
AKZO NOBEL N.V. [NL/NL]; Velperweg 76 NL-6824 BM Arnhem, NL (AT, BE, CA, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, JP, KR, LU, MC, NL, PT, SE)
BRAKENHOFF, Godefriedus, Jacobus [NL/NL]; NL (UsOnly)
GHAUHARALI, Rick, Iftekar [NL/NL]; NL (UsOnly)
HOFSTRAAT, Johannes, Willem [NL/NL]; NL (UsOnly)
Inventors:
BRAKENHOFF, Godefriedus, Jacobus; NL
GHAUHARALI, Rick, Iftekar; NL
HOFSTRAAT, Johannes, Willem; NL
Agent:
SCHALKWIJK, Pieter, Cornelis; Akzo Nobel N.V. Patent Dept. (Dept. APTA) P.O. Box 9300 NL-6800 SB Arnhem, NL
Priority Data:
97201235.525.04.1997EP
Title (EN) PHOTOBLEACHABLE LUMINESCENT LAYERS FOR CALIBRATION AND STANDARDIZATION IN OPTICAL MICROSCOPY
(FR) COUCHES LUMINESCENTES POUVANT ETRE PHOTOBLANCHIES POUR LE CALIBRAGE ET L'ETALONNAGE EN MICROSCOPIE OPTIQUE
Abstract:
(EN) The invention pertains to a calibration layer comprising an optically transparent polymer containing an amount of photobleachable luminscent material present in such a way that the final polymer film contains less than 10 wt.% of luminophore and has an optical attenuation of less than 0.3 absorption units in the wavelength region of interest. The invention further is concerned with a method of calibration of an optical image device, preferably an optical or Raman microscope, by using the decrease in luminescence as the result of photobleaching between two consecutive images for calibration.
(FR) L'invention concerne une couche de calibrage qui comprend un polymère optiquement transparent. Ce polymère contient une quantité d'un matériau luminescent pouvant être photoblanchi de telle sorte que le film polymère final contient moins de 10 % en poids de luminophore, et présente une atténuation optique inférieure à 0,3 unités d'absorption dans la zone de longueur d'ondes d'intérêt. L'invention traite, en outre, d'un procédé de calibrage d'un dispositif à images optiques, de préférence, un microscope optique ou Raman utilisant la diminution de la luminescence comme résultat du photoblanchiment entre deux images consécutives pour le calibrage.
Designated States: CA, JP, KR, US
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0977981US6259524