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1. (WO1998049537) PHOTOBLEACHABLE LUMINESCENT LAYERS FOR CALIBRATION AND STANDARDIZATION IN OPTICAL MICROSCOPY

Pub. No.:    WO/1998/049537    International Application No.:    PCT/EP1998/002358
Publication Date: Fri Nov 06 00:59:59 CET 1998 International Filing Date: Sat Apr 18 01:59:59 CEST 1998
IPC: G01N 21/64
G01N 21/65
Applicants: AKZO NOBEL N.V.
BRAKENHOFF, Godefriedus, Jacobus
GHAUHARALI, Rick, Iftekar
HOFSTRAAT, Johannes, Willem
Inventors: BRAKENHOFF, Godefriedus, Jacobus
GHAUHARALI, Rick, Iftekar
HOFSTRAAT, Johannes, Willem
Title: PHOTOBLEACHABLE LUMINESCENT LAYERS FOR CALIBRATION AND STANDARDIZATION IN OPTICAL MICROSCOPY
Abstract:
The invention pertains to a calibration layer comprising an optically transparent polymer containing an amount of photobleachable luminscent material present in such a way that the final polymer film contains less than 10 wt.% of luminophore and has an optical attenuation of less than 0.3 absorption units in the wavelength region of interest. The invention further is concerned with a method of calibration of an optical image device, preferably an optical or Raman microscope, by using the decrease in luminescence as the result of photobleaching between two consecutive images for calibration.