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1. (WO1998049345) METHODS AND COMPOSITIONS FOR TARGETED DNA DIFFERENTIAL DISPLAY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/049345 International Application No.: PCT/US1998/008616
Publication Date: 05.11.1998 International Filing Date: 29.04.1998
Chapter 2 Demand Filed: 23.11.1998
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
TRUSTEES OF BOSTON UNIVERSITY [US/US]; 147 Bay State Road Boston, MA 02215, US (AL, AM, AT, AU, AZ, BB, BE, BF, BG, BJ, BR, BY, CA, CF, CG, CH, CI, CM, CN, CY, CZ, DE, DK, EE, ES, FI, FR, GA, GB, GE, GH, GM, GN, GR, GW, HU, ID, IE, IL, IS, IT, JP, KE, KG, KP, KR, KZ, LK, LR, LS, LT, LU, LV, MC, MD, MG, MK, ML, MN, MR, MW, MX, NE, NL, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, SN, SZ, TD, TG, TJ, TM, TR, TT, UA, UG, UZ, VN, YU, ZW)
SMITH, Cassandra, L. [US/US]; US (UsOnly)
Inventors:
SMITH, Cassandra, L.; US
Agent:
CARROLL, Peter, G. ; Medlen & Carroll, LLP Suite 2200 220 Montgomery Street San Francisco, CA 94104, US
Priority Data:
60/045,07829.04.1997US
Title (EN) METHODS AND COMPOSITIONS FOR TARGETED DNA DIFFERENTIAL DISPLAY
(FR) PROCEDES ET COMPOSITIONS UTILES POUR L'AFFICHAGE DIFFERENTIEL D'ADN CIBLES
Abstract:
(EN) Methods and compositions are described for distinguishing between the expression of genes in two or more biological samples. In one embodiment, a PCR-based genomic differential display method is described. The method uses PCR suppression to reduce genome complexity and to amplify genomic fragments containing a targeted interspersed repeat sequence. The PCR products can be displayed by size to create a genomic fingerprint or cloned to create a library of fragments containing the targeted repeat sequence and their flanking sequences. The primers and method of the present invention allows for the accurate identification of differentially expressed gene(s) in various cell types.
(FR) L'invention concerne des procédés et des compositions permettant de différencier l'expression de gènes dans deux ou davantage d'échantillons biologiques. Dans un mode de réalisation, un procédé d'affichage différentiel génomique employant une PCR est décrit. Le procédé emploie une suppression par PCR pour réduire la complexité du génome et pour amplifier des fragments génomiques contenant une séquence de répétition intercalée ciblée. Les produits de PCR peuvent être affichés selon leur taille pour produire une empreinte génomique, ou clonés pour produire une banque de fragments contenant la séquence de répétition ciblée et leurs séquences flanquantes. Les amorces et le procédé de la présente invention permettent l'identification précise de gènes exprimés de façon différente dans divers types de cellules.
Designated States: AL, AM, AT, AU, AZ, BB, BG, BR, BY, CA, CH, CN, CZ, DE, DK, EE, ES, FI, GB, GE, GH, GM, GW, HU, ID, IL, IS, JP, KE, KG, KP, KR, KZ, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, UA, UG, US, UZ, VN, YU, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, SD, SZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU1998072652