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1. (WO1998049341) POLYNUCLEOTIDE SEQUENCING USING SEMI-DEGENERATE PRIMERS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/049341 International Application No.: PCT/GB1998/001233
Publication Date: 05.11.1998 International Filing Date: 28.04.1998
Chapter 2 Demand Filed: 19.11.1998
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
WEBSTER, Andrew [GB/US]; GB
Inventors:
WEBSTER, Andrew; GB
Agent:
GILL JENNINGS & EVERY; Broadgate House 7 Eldon Street London EC2M 7LH, GB
Priority Data:
9708606.028.04.1997GB
Title (EN) POLYNUCLEOTIDE SEQUENCING USING SEMI-DEGENERATE PRIMERS
(FR) SEQUENÇAGE D'UN POLYNUCLEOTIDE AU MOYEN D'AMORCES SEMIDEGENEREES
Abstract:
(EN) A method for the analysis of polynucleotide molecules comprises the steps of: a) reacting a target polynucleotide with oligonucleotide primers, a polymerase enzyme, and the other reagents necessary for the polymerase reaction, wherein the oligonucleotide primers are chosen such that polymerase products of varying lengths are produced; and b) analysing the products of the said reaction or reactions; wherein the oligonucleotide primers include an array of semi-degenerate primers whose (3') ends comprise variations of one or more nucleotides A, T, G, and C such that the array is complementary to all the polynucleotide sequence.
(FR) L'invention concerne un procédé d'analyse de molécules polynucléotidiques. Ce procédé comprend les étapes consistant: a) à faire réagir un polynucléotide cible avec des amorces nucléotidiques, une polymérase, et les autres réactifs nécessaires pour obtenir une réaction de la polymérase, lesdites amorces nucléotidiques étant choisies de sorte que des produits de la polymérase de longueur variable puissent être générés; b) à analyser les produits de la réaction ou des réactions susmentionnée(s), les amorces nucléotidiques renfermant un ensemble d'amorces semidégénérées dont les extrémités (3') comprennent des variations d'un ou plusieurs nucléotides A, T, G, et C, de sorte que ledit ensemble est complémentaire à toute la séquence polynucléotidique.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, GH, GM, GW, HU, ID, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, UA, UG, US, UZ, VN, YU, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, SD, SZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0979307AU1998072203