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1. (WO1998049328) IMPROVED PROKARYOTIC EXPRESSION OF PROTEIN
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/049328 International Application No.: PCT/GB1998/001051
Publication Date: 05.11.1998 International Filing Date: 24.04.1998
Chapter 2 Demand Filed: 28.10.1998
IPC:
C12N 9/28 (2006.01) ,C12N 9/56 (2006.01) ,C12N 15/75 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
26
acting on alpha-1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
28
Alpha-amylase from microbial source, e.g. bacterial amylase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
48
acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50
Proteinases
52
derived from bacteria
54
bacteria being Bacillus
56
Bacillus subtilis or Bacillus licheniformis
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75
for Bacillus
Applicants:
THE UNIVERSITY OF NEWCASTLE [GB/GB]; 18 Windsor Terrace Jesmond Newcastle upon Tyne NE2 4HE, GB (AL, AM, AT, AU, AZ, BA, BB, BE, BF, BG, BJ, BR, BY, CA, CF, CG, CH, CI, CM, CN, CU, CY, CZ, DE, DK, EE, ES, FI, FR, GA, GB, GE, GH, GM, GN, GR, GW, HU, ID, IE, IL, IS, IT, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MC, MD, MG, MK, ML, MN, MR, MW, MX, NE, NL, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, SN, SZ, TD, TG, TJ, TM, TR, TT, UA, UG, UZ, VN, YU, ZW)
NOVO NORDISK A/S [DK/DK]; Novo Allé DK-2880 Bagsværd, DK (AL, AM, AT, AU, AZ, BA, BB, BE, BF, BG, BJ, BR, BY, CA, CF, CG, CH, CI, CM, CN, CU, CY, CZ, DE, DK, EE, ES, FI, FR, GA, GB, GE, GH, GM, GN, GR, GW, HU, ID, IE, IL, IS, IT, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MC, MD, MG, MK, ML, MN, MR, MW, MX, NE, NL, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, SN, SZ, TD, TG, TJ, TM, TR, TT, UA, UG, UZ, VN, YU, ZW)
HARWOOD, Colin, Robert [GB/GB]; GB (UsOnly)
STEPHENSON, Keith [GB/GB]; GB (UsOnly)
JORGENSEN, Steen [DK/DK]; DK (UsOnly)
JENSEN, Christina [DK/DK]; DK (UsOnly)
KRISTENSEN, Tina [DK/DK]; DK (UsOnly)
Inventors:
HARWOOD, Colin, Robert; GB
STEPHENSON, Keith; GB
JORGENSEN, Steen; DK
JENSEN, Christina; DK
KRISTENSEN, Tina; DK
Priority Data:
9708452.926.04.1997GB
Title (EN) IMPROVED PROKARYOTIC EXPRESSION OF PROTEIN
(FR) EXPRESSION PROCARYOTIQUE AMELIOREE DE PROTEINES
Abstract:
(EN) The invention herein described relates to a novel prokaryotic expression system and proteins expressed thereby. The regulated expression or alteration of the $i(wprA) gene from the genome of $i(B.subtilis) has been shown to result in enhanced secretion of selected native, heterologous or recombinant polypeptides. This alteration provides the means by which the yield of secreted polypeptide is increased compared to strains carrying a wild type copy of the $i(wprA) gene.
(FR) L'invention concerne un nouveau système d'expression procaryotique et les protéines exprimées par ce système. On a montré que l'expression ou la modification du gène $i(wprA) du génome de $i(B.subtilis) entraîne une sécrétion accrue de polypeptides natifs sélectionnés hétérologues ou recombinés. Cette modification permet d'augmenter le rendement de polypeptides secrétés par rapport aux souches portant une copie de type sauvage du gène $i(wprA).
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, GH, GM, GW, HU, ID, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, UA, UG, US, UZ, VN, YU, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, SD, SZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0977875JP2001527401 CN1253591DE000069837086AU1998070570DK0977875