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1. (WO1998049325) REPORTER FUSION PROTEINS, EXPRESSION VECTORS AND TRANSFECTED CELL LINES THEREOF FOR THE ANALYSIS OF NUCLEAR TRANSPORT
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/1998/049325 International Application No.: PCT/CA1998/000385
Publication Date: 05.11.1998 International Filing Date: 24.04.1998
Chapter 2 Demand Filed: 26.11.1998
IPC:
C07K 14/435 (2006.01) ,C07K 14/46 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
46
from vertebrates
Applicants:
STOCHAJ, Ursula [DE/CA]; CA (UsOnly)
McGILL UNIVERSITY [CA/CA]; 845 Sherbrooke Street West Montréal, Québec H3A 1B1, CA (AL, AM, AT, AU, AZ, BA, BB, BE, BF, BG, BJ, BR, BY, CA, CF, CG, CH, CI, CM, CN, CU, CY, CZ, DE, DK, EE, ES, FI, FR, GA, GB, GE, GH, GM, GN, GR, GW, HU, ID, IE, IL, IS, IT, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MC, MD, MG, MK, ML, MN, MR, MW, MX, NE, NL, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, SN, SZ, TD, TG, TJ, TM, TR, TT, UA, UG, UZ, VN, YU, ZW)
Inventors:
STOCHAJ, Ursula; CA
Agent:
COTÉ, France; Swabey Ogilvy Renault Suite 1600 1981 McGill College Avenue Montréal, Québec H3A 2Y3, CA
Priority Data:
60/044,91128.04.1997US
Title (EN) REPORTER FUSION PROTEINS, EXPRESSION VECTORS AND TRANSFECTED CELL LINES THEREOF FOR THE ANALYSIS OF NUCLEAR TRANSPORT
(FR) PROTEINES HYBRIDES DE MARQUAGE, VECTEURS D'EXPRESSION ET LIGNEES CELLULAIRES TRANSFECTEES DE CES PROTEINES DESTINES A L'ANALYSE DU TRANSPORT DU NOYAU
Abstract:
(EN) The present invention relates to reporter fusion proteins which allow the monitoring of facilitated transport and passive diffusion of proteins across the nuclear envelope in mammalian cells, cloning and expression vectors encoding these reporter proteins and transfected cell lines thereof. More particularly, the present invention relates to genes encoding reporter proteins to monitor transport and passive diffusion of proteins across the nuclear envelope in mammalian cells, which comprises a protein carrying a nuclear localization sequence (NLS) fused to at least one copy of a reporter protein, wherein the reporter protein allows for the visualization of the reporter fusion protein.
(FR) La présente invention se rapporte à des protéines hybrides marqueurs qui permettent le contrôle du transport facilité et de la diffusion passive de protéines à travers la membrane nucléaire de cellules de mammifères; au clonage et à des vecteurs d'expression codant ces protéines marqueurs et les lignées cellulaires transfectées de ces protéines. La présente invention concerne plus particulièrement des gènes codant pour des protéines marqueurs en vue de contrôler le transport et la diffusion passive de protéines à travers la membrane nucléaire de cellules de mammifères qui comporte une protéine portant une séquence de localisation du noyau (NLS) qui a fusionné en au moins une copie d'une protéine marqueur. La protéine marqueur permet la visualisation de la protéine hybride marqueur.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, GH, GM, GW, HU, ID, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, UA, UG, US, UZ, VN, YU, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, SD, SZ, UG, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)