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1. (WO1998049273) RAFFINOSE SYNTHETASE GENE, PROCESS FOR PRODUCING RAFFINOSE, AND TRANSFORMED PLANT
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/049273 International Application No.: PCT/JP1997/003879
Publication Date: 05.11.1998 International Filing Date: 24.10.1997
Chapter 2 Demand Filed: 24.10.1997
IPC:
C12N 9/10 (2006.01) ,C12N 15/82 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10
Transferases (2.)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
Applicants:
AJINOMOTO CO., INC. [JP/JP]; 15-1, Kyobashi 1-chome Chuo-ku Tokyo 104, JP (AllExceptUS)
OSUMI, Chieko [JP/JP]; JP (UsOnly)
NOZAKI, Jinshi [JP/JP]; JP (UsOnly)
KIDA, Takao [JP/JP]; JP (UsOnly)
Inventors:
OSUMI, Chieko; JP
NOZAKI, Jinshi; JP
KIDA, Takao; JP
Agent:
TOYAMA, Tsutomu ; Yokoyama Building 6th floor 4-10, Higashi Nihonbashi 3-chome Chuo-ku Tokyo 103, JP
Priority Data:
9/11112428.04.1997JP
Title (EN) RAFFINOSE SYNTHETASE GENE, PROCESS FOR PRODUCING RAFFINOSE, AND TRANSFORMED PLANT
(FR) GENE DE RAFFINOSE SYNTHETASE, PROCEDE DE PRODUCTION DE RAFFINOSE, ET PLANTE TRANSFORMEE
Abstract:
(EN) A raffinose is produced by the action of a raffinose synthetase having the following properties on sucrose and galactinol: (1) activity and substrate specificity: raffinose is produced from sucrose and galactinol; (2) optimal pH: about 6-8; (3) optimal temp.: about 35-40 °C; (4) molecular weight: (i) molecular weight as measured by gel filtration chromatography: about 75-95 kDa; (ii) molecular weight as measured by polyacrylamide gel electrophoresis (native PAGE): about 90-100 kDa; and (iii) molecular weight as measured by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions: about 90-100 kDa; (5) inhibition: it is inhibited by iodoacetamide, N-ethylmaleimide, and myoinositol.
(FR) Selon l'invention, une raffinose est produite par l'action sur du sucrose et du galactinol d'une raffinose synthétase ayant les propriétés suivantes: (1) activité et spécificité du substrat: la raffinose est produite à partir de succrose et de galactinol; (2) pH optimal: environ 6-8; (3) température optimale: environ 35-40 °C; (4) poids moléculaire: (i) poids moléculaire mesuré par chromatographie sur gel : environ 75-95 kDa; (ii) poids moléculaire mesuré par électrophorèse en gel de polyacrylamide naturel : environ 90-100 kDa; et (iii) poids moléculaire mesuré par électrophorèse en gel de polyacrylamide en présence de dodécyl sulfate de sodium, dans des conditions réductrices : environ 90-100 kDa; (5) inhibition: par iodoacétamide, N-éthylmaleimide et myoinositol.
Designated States: AU, BR, CA, CN, US
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
BRPI9714675EP0994186US20050118300US6891084US20050066391US20050160497
CN1259996CA2287914AU1998081874