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1. (WO1998049180) TRIPLEX FORMING OLIGONUCLEOTIDES INCLUDING PYRAZOLO(3,4-D)PYRIMIDINE BASES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/049180 International Application No.: PCT/US1998/008373
Publication Date: 05.11.1998 International Filing Date: 29.04.1998
Chapter 2 Demand Filed: 04.11.1998
IPC:
C07H 21/00 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
EPOCH PHARMACEUTICALS, INC. [US/US]; 1725 - 220th Street, S.E. #104 Bothell, WA 98021, US
Inventors:
MEYER, Rich, B.; US
GALL, Alexander; US
KUTYAVIN, Igor, V.; US
Agent:
KLEIN, Howard, J. ; Klein & Szekeres, LLP Suite 700 4199 Campus Drive Irvine, CA 92612, US
Priority Data:
08/848,37330.04.1997US
Title (EN) TRIPLEX FORMING OLIGONUCLEOTIDES INCLUDING PYRAZOLO(3,4-D)PYRIMIDINE BASES
(FR) OLIGONUCLEOTIDES EN TRIPLEX, INCLUANT DES BASES PYRAZOLO(3,4-D)PYRIMIDINE
Abstract:
(EN) A triplex forming oligonucleotide is complementary pursuant to the G/T or A/G recognition motif to a homopurine, or substantially homopurine target sequence in double stranded nucleic acids, and at least one and preferably all of the guanine bases are replaced by their pyrazolo[3,4-d]pyrimidine analog, namely by 6-amino-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one. The oliginucleotides containing the pyrazolo[3,4-d]pyrimidine analog of guanine exhibit a lesser degree of self-association, and lack the nucleophilic nitrogen atom in the 7 position of guanine. The latter feature results in a diminished extent of self-cross-linking in ODNs which also have a covalently attached cross-linking agent.
(FR) Par rapport au motif de reconnaissance G/T ou A/G, un tel oligonucléotide en triplex est complémentaire d'une homopurine ou d'une séquence cible sensiblement homopurine d'acides nucléiques à double brin, l'une au moins, mais de préférence la totalité, des bases guanine étant remplacées par l'analogue pyrazolo[3,4-d]pyrimidine correspondant, en l'occurrence le 6-amino-1$i(H)-pyrazolo[3,4-d]pyrimidin-4(5$i(H))-one. Les oligonucléotides contenant l'analogue pyrazolo[3,4-d]pyrimidine de guanine font preuve d'un moindre degré d'auto-association et ne comportent par l'atome d'azote nucléophile en position 7 de la guanine. Cette dernière caractéristique aboutit à un moindre niveau d'auto-réticulation des oligonucléotides auxquels est attaché par covalence un agent de réticulation.
Designated States: AL, AM, AU, AZ, BA, BB, BG, BR, BY, CA, CN, CU, CZ, EE, FI, GE, GH, GM, GW, HU, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, RO, RU, SD, SG, SI, SK, SL, TJ, TM, TR, TT, UA, UG, UZ, VN, YU, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, SD, SZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU1998072586