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1. (WO1998049176) GLYCOCONJUGATED FLUORESCENT LABELING REAGENTS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1998/049176 International Application No.: PCT/US1998/006372
Publication Date: 05.11.1998 International Filing Date: 02.04.1998
Chapter 2 Demand Filed: 23.09.1998
IPC:
C07H 17/02 (2006.01) ,C08B 37/02 (2006.01) ,C08B 37/16 (2006.01) ,C09B 23/02 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 33/533 (2006.01) ,G01N 33/58 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
17
Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
02
Heterocyclic radicals containing only nitrogen as ring hetero atoms
C CHEMISTRY; METALLURGY
08
ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
B
POLYSACCHARIDES; DERIVATIVES THEREOF
37
Preparation of polysaccharides not provided for in groups C08B1/-C08B35/110; Derivatives thereof
02
Dextran; Derivatives thereof
C CHEMISTRY; METALLURGY
08
ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
B
POLYSACCHARIDES; DERIVATIVES THEREOF
37
Preparation of polysaccharides not provided for in groups C08B1/-C08B35/110; Derivatives thereof
16
Cyclodextrin; Derivatives thereof
C CHEMISTRY; METALLURGY
09
DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
B
ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES; MORDANTS; LAKES
23
Methine or polymethine dyes, e.g. cyanine dyes
01
characterised by the methine chain
02
containing an odd number of CH groups
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
531
Production of immunochemical test materials
532
Production of labelled immunochemicals
533
with fluorescent label
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58
involving labelled substances
Applicants:
CARNEGIE MELLON UNIVERSITY [US/US]; 5000 Forbes Avenue Pittsburgh, PA 15213, US
Inventors:
REDDINGTON, Mark, V.; US
WAGGONER, Alan, S.; US
Agent:
CRAWFORD, Arthur, R.; Nixon & Vanderhye P.C. 8th floor 1100 North Glebe Road Arlington, VA 22201-4714, US
Priority Data:
08/847,48225.04.1997US
Title (EN) GLYCOCONJUGATED FLUORESCENT LABELING REAGENTS
(FR) REACTIFS DE MARQUAGE FLUORESCENTS GLYCOCONJUGUES
Abstract:
(EN) Water soluble glycoconjugated fluorescent labeling reagents comprised of a fluorescent dye, one or more sugar or carbohydrate residues that impart water solubility, and a reactive group that allows covalent attachment of the reagent to a substrate are disclosed. Glyconjugated fluorescent labeling reagents having formula (I). These glycoconjugates are highly water soluble because of the sugar residues. This property is expected to lower nonspecific binding to cellular matter, reduce precipitation of labeled substrates, inhibit quenching of fluorescence, and unlike many prior fluorescent labeling reagents, that are soluble only in organic solvents, render the glycoconjugates usable in wholly aqueous solution.
(FR) L'invention concerne des réactifs de marquage fluorescents glycoconjugués, comprenant un colorant fluorescent, un ou plusieurs résidus de sucre ou de glucide conférant la solubilité dans l'eau, et un groupe réactif qui permet la liaison de covalence entre le réactif et un substrat. Lesdits réactifs sont représentés par la formule (I). Ces glycoconjugués sont très hydrosolubles en raison de la présence des résidus de sucre. On attend de la solubilité en question qu'elle diminue la liaison non spécifique sur matière cellulaire, réduise la précipitation des substrats marqués, inhibe l'extinction de fluorescence et, contrairement à beaucoup de réactifs de marquage fluorescents existants qui sont solubles uniquement dans des solvants organiques, rende les glycoconjugués utilisables dans des solutions entièrement aqueuses.
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Designated States: AU, CA, JP
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0977766JP2001524969 CA2288275AU1998068746