Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO1998012351) METHOD FOR PURIFYING AND EVENTUALLY ANALYZING NUCLEIC ACIDS FROM BIOLOGICAL TEST SAMPLES
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/1998/012351 International Application No.: PCT/EP1997/005129
Publication Date: 26.03.1998 International Filing Date: 18.09.1997
Chapter 2 Demand Filed: 15.04.1998
IPC:
C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
ROTH, W., Kurt [DE/DE]; DE
WASCHK, Dorothee [DE/DE]; DE
ZEUZEM, Stefan [DE/DE]; DE
Inventors:
ROTH, W., Kurt; DE
WASCHK, Dorothee; DE
ZEUZEM, Stefan; DE
Agent:
PELLMANN, Hans-Bernd; Tiedtke-Bühling-Kinne Bavariaring 4 D-80336 München, DE
Priority Data:
196 38 362.519.09.1996DE
197 31 670.023.07.1997DE
Title (EN) METHOD FOR PURIFYING AND EVENTUALLY ANALYZING NUCLEIC ACIDS FROM BIOLOGICAL TEST SAMPLES
(FR) PROCEDE DE PURIFICATION ET EVENTUELLEMENT D'ANALYSE D'ACIDES NUCLEIQUES CONTENUS DANS DES ECHANTILLONS BIOLOGIQUES
(DE) VERFAHREN ZUR REINIGUNG UND GEGEBENENFALLS ANALYSE VON NUKLEINSÄUREN AUS BIOLOGISCHEN PROBEN
Abstract:
(EN) The invention concerns a method for purifying and eventually analyzing nucleic acids from biological test samples. In said method the test sample containing nucleic acid is made to react with an anion-exchanging synthetic resin, which has a preferably high bonding affinity with bile acid. The possible existing inhibitors for the eventually ensuing nucleic acid analysis reaction are bonded and separated by the anion-exchanging synthetic resin.
(FR) L'invention concerne un procédé de purification et éventuellement d'analyse d'acides nucléiques contenus dans des échantillons biologiques, ledit procédé consistant à faire réagir l'échantillon contenant l'acide nucléique avec une résine synthétique échangeuse d'anions, possédant une affinité de liaison, de préférence élevée, avec les acides biliaires. Les inhibiteurs éventuellement présents pour la réaction d'analyse éventuellement effectuée ensuite, sont liés à la résine échangeuse d'anions puis séparés.
(DE) Es wird ein Verfahren zur Reinigung und gegebenenfalls Analyse von Nukleinsäuren aus biologischen Proben beschrieben, wobei das Verfahren den Schritt umfaßt, daß die Nukleinsäure-haltige Probe mit einem Anionenaustauscher-Kunstharz umgesetzt wird, welches eine, vorzugsweise hohe, Bindungsaffinität zu Gallensäuren besitzt, wobei die möglicherweise vorhandenen Inhibitoren für die gegebenenfalls anschließende Nukleinsäure-Analysereaktion an das Anionenaustauscherharz gebunden und abgetrennt werden.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, GH, HU, ID, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, UA, UG, US, UZ, VN, YU, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, KE, LS, MW, SD, SZ, UG, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: German (DE)
Filing Language: German (DE)