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1. WO1997046716 - METHOD TO DETECT HCV SPECIFIC NUCLEIC ACIDS

Publication Number WO/1997/046716
Publication Date 11.12.1997
International Application No. PCT/IT1997/000128
International Filing Date 03.06.1997
Chapter 2 Demand Filed 24.11.1997
IPC
C12Q 1/70 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
CPC
C12Q 1/707
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
701Specific hybridization probes
706for hepatitis
707non-A, non-B Hepatitis, excluding hepatitis D
Applicants
  • WABCO B.V. [NL]/[NL] (AllExceptUS)
  • BOSIO, Paolo [IT]/[IT] (UsOnly)
  • STRUMIA, Claudia [IT]/[IT] (UsOnly)
  • CLEMENZA, Filippo [IT]/[IT] (UsOnly)
Inventors
  • BOSIO, Paolo
  • STRUMIA, Claudia
  • CLEMENZA, Filippo
Agents
  • BANCHETTI, Marina
Priority Data
RM96A00040407.06.1996IT
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD TO DETECT HCV SPECIFIC NUCLEIC ACIDS
(FR) PROCEDE POUR DETECTER DES ACIDES NUCLEIQUES SPECIFIQUES DU VHC
Abstract
(EN)
A process to detect hepatitis C virus (HCV) specific nucleic acids comprising the steps of: (a) reverse transcribing the viral RNA by means of a primer having specified sequences; (b) amplifying by a single polymerase chain reaction (PCR) with a specific primer; wherein the Mg++/Taq polymerase ratio in the reaction mix is of approx. 100 nmoles/enzyme unit; (c) revealing the amplified product by means of the DEIA method using a specific probe.
(FR)
Cette inventio se rapporte à un procédé pour détecter des acides nucléiques spécifiques du virus de l'hépatite C (VHC), qui consiste: a) à effectuer une transcription inverse de l'ARN viral au moyen d'une amorce ayant des séquences spécifiées; b) à effectuer une amplification par une réaction en chaîne de polymérase unique (PCR) au moyen d'une amorce spécifique; où le rapport Mg++/Taq-polymérase dans le mélange de réaction est approximativement égale à 100 nmoles/unité d'enzyme; c) et à révéler le produit amplifié au moyen d'un procédé de dosage immunoenzymatique d'ADN, en utilisant une sonde spécifique.
Also published as
Latest bibliographic data on file with the International Bureau