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Machine translation
1. (WO1997038090) A CULTURAL MEDIUM FOR MAINTAINING NEURAL CELLS IN AMBIENT ATMOSPHERE
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1997/038090    International Application No.:    PCT/US1997/005875
Publication Date: 16.10.1997 International Filing Date: 09.04.1997
Chapter 2 Demand Filed:    06.11.1997    
IPC:
C12N 5/02 (2006.01), C12N 5/0793 (2010.01)
Applicants: BOARD OF THE TRUSTEES OF SOUTHERN ILLINOIS UNIVERSITY [US/US]; 801 North Rutledge Street, Springfield, IL 62703 (US) (For All Designated States Except US).
BREWER, Gregory, J. [US/US]; (US) (For US Only).
PRINCE, Paul, J. [US/US]; (US) (For US Only)
Inventors: BREWER, Gregory, J.; (US).
PRINCE, Paul, J.; (US)
Agent: NORTHRUP, Thomas, E.; Dressler, Rockey, Milnamow & Katz, Ltd., Suite 4700, Two Prudential Plaza, Chicago, IL 60601 (US)
Priority Data:
60/015,049 09.04.1996 US
Title (EN) A CULTURAL MEDIUM FOR MAINTAINING NEURAL CELLS IN AMBIENT ATMOSPHERE
(FR) MILIEU DE CULTURE PERMETTANT LA CONSERVATION DE CELLULES NEURALES EN ATMOSPHERE AMBIANTE
Abstract: front page image
(EN)The present invention provides a minimal essential medium for maintaining neural cell or tissue viability in an environment containing ambient levels of CO¿2?. The medium contains less than about 2000 $g(m)M bicarbonate, has an osmolality of from about 230 mOsm to about 300 mOsm, contains a buffer having a pKa of from about 6.9 to about 7.7, and is free of ferrous sulfate, glutamate and aspartate. The present invention also provides a process of extending neural cell or tissue viability in an atmosphere having ambient levels of carbon dioxide whereby the neural cells or tissue are placed in such a medium. Preferably, the medium is supplemented with a growth-promoting medium that contains effective amounts of hormones, essential fatty acids and anti-oxidants for neural cells.
(FR)La présente invention se rapporte à un milieu de culture minimum nécessaire au maintien de la viabilité de cellules neurales ou de tissus nerveux dans un environnement contenant des niveaux ambiants de CO¿2?. Ledit milieu contient moins de 2000 $g(m)M environ de bicarbonate, possède une osmolalité comprise entre 230 mOsm environ et 300 mOsm environ, contient un tampon ayant un pKa compris entre 6,9 environ et 7,7 environ, et il ne contient ni sulfate ferreux, ni glutamate ni aspartate. La présente invention se rapporte également à un procédé permettant d'accroître la viabilité de cellules neurales ou de tissus nerveux dans une atmosphère ayant des niveaux ambiants de dioxyde de carbone et consistant à placer les cellules neurales ou les tissus nerveux dans un tel milieu. De préférence, on ajoute à ce milieu un milieu favorisant la croissance qui contient des quantités efficaces d'hormones, d'acides gras essentiels et d'antioxydants destinés aux cellules neurales.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, US, UZ, VN.
African Regional Intellectual Property Organization (GH, KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)