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1. WO1997031259 - NON-CAPTIVE SUBSTRATE LIQUID PHASE IMMUNOASSAY

Publication Number WO/1997/031259
Publication Date 28.08.1997
International Application No. PCT/US1997/002155
International Filing Date 21.02.1997
Chapter 2 Demand Filed 22.09.1997
IPC
B01L 3/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
G01N 33/538 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
537with separation of immune complex from unbound antigen or antibody
538by sorbent column, particles or resin strip
G01N 33/58 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
CPC
B01L 2300/0825
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0809rectangular shaped
0825Test strips
B01L 2400/0406
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
04Moving fluids with specific forces or mechanical means
0403specific forces
0406capillary forces
B01L 3/5023
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5023with a sample being transported to, and subsequently stored in an absorbent for analysis
G01N 2333/4737
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
435from animals; from humans
46from vertebrates
47Assays involving proteins of known structure or function as defined in the subgroups
4701Details
4737C-reactive protein
G01N 33/538
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
537with separation of immune complex from unbound antigen or antibody
538by sorbent column, particles or resin strip ; , i.e. sorbent materials
G01N 33/585
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
585with a particulate label, e.g. coloured latex
Applicants
  • DEXALL BIOMEDICAL LABS, INC. [US]/[US] (AllExceptUS)
  • HUBSCHER, Thomas, T. [US]/[US] (UsOnly)
  • LILLEHOJ, Erik, P. [US]/[US] (UsOnly)
Inventors
  • HUBSCHER, Thomas, T.
  • LILLEHOJ, Erik, P.
Agents
  • EDELL, Ira, C.
Priority Data
08/605,59522.02.1996US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) NON-CAPTIVE SUBSTRATE LIQUID PHASE IMMUNOASSAY
(FR) DOSAGE IMMUNOLOGIQUE EN PHASE LIQUIDE D'UN SUBSTRAT NON CAPTIF
Abstract
(EN)
An immunoassay of a liquid biological specimen for a specific analyte is conducted by forming a test sample by contacting the specimen with: (1) a binding substance of a ligand, antiligand or receptor capable of binding the analyte and (2) a detector substance of a colloidal gold or silver labeled ligand or antiligand to form a test sample. The test sample is applied by flowing onto a defined zone of an insoluble porous support film having a pore size impassable to a complex formed between the analyte, if present, with the binding substance and the detector substance, but passable to the binding substance and detector substance while remaining uncomplexed in the absence of the desired analyte. If the analyte is present in the test specimen, the detector substance binds with the analyte and the binding substance to form a visually inspectable complex on the surface of the porous support film. After application of the test sample to the porous support, the surface of the porous support is visually inspected for color to determine the presence and quantity or the absence of the analyte being assayed.
(FR)
On procède à un dosage immunologique d'un prélèvement biologique de liquide pour un analyte spécifique en constituant un échantillon d'essai par la mise en contact du prélèvement avec 1), une substance de liaison d'un ligand, d'un anti-ligand ou d'un récepteur capable de fixer l'analyte et 2), une substance de détection d'un ligand ou d'un anti-ligand marqué à l'or ou à l'argent colloïdaux, pour constituer l'échantillon d'essai. Ce dernier est déposé, par écoulement, sur une zone définie d'un film support poreux insoluble que la taille de ses pores rend infranchissable à un complexe formé de la substance de liaison et de la substance de détection ainsi que de l'analyte, s'il est présent, mais qui laisse passer les substances de liaison et de détection, celles-ci ne formant pas de complexe en l'absence de l'analyte souhaité. Si l'analyte est présent dans l'échantillon d'essai, la substance de détection se lie à celui-ci ainsi qu'à la substance de liaison pour donner un complexe, qu'il est possible d'examiner visuellement, à la surface du film support poreux. Après avoir déposé l'échantillon d'essai sur le support poreux, on en inspecte visuellement la surface dont la couleur permet de déterminer l'absence ou la présence de l'analyte en cours de dosage et, si celui-ci est présent, sous quelles quantités.
Also published as
NO19983854
Latest bibliographic data on file with the International Bureau