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1. WO1997030150 - MOLECULES FOR THE INDUCTION OF IMMUNOLOGICAL TOLERANCE

Publication Number WO/1997/030150
Publication Date 21.08.1997
International Application No. PCT/EP1997/000777
International Filing Date 17.02.1997
Chapter 2 Demand Filed 15.09.1997
IPC
A61K 38/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C07K 14/47 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
C12N 15/01 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
01Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
CPC
A61K 38/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C07K 14/4713
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
4701not used
4713Autoimmune diseases, e.g. Insulin-dependent diabetes mellitus, multiple sclerosis, rheumathoid arthritis, systemic lupus erythematosus; Autoantigens
C12N 15/102
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
102Mutagenizing nucleic acids
C12N 15/1058
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
Applicants
  • PANGENETICS B.V. [NL]/[NL] (AllExceptUS)
  • BALINT, Robert, F. [US]/[US] (UsOnly)
  • WAUBEN, Marca, Henriëtte, Michaela [NL]/[NL] (UsOnly)
Inventors
  • BALINT, Robert, F.
  • WAUBEN, Marca, Henriëtte, Michaela
Agents
  • DE BRUIJN, Leendert, C.
Priority Data
96200383.615.02.1996EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MOLECULES FOR THE INDUCTION OF IMMUNOLOGICAL TOLERANCE
(FR) MOLECULES INDUISANT UNE TOLERANCE IMMUNOLOGIQUE
Abstract
(EN)
The invention relates to a population of protein molecules having a distribution of specific mutations in the amino acid sequence as compared to a parent protein, which population of protein molecules is obtainable by establishing the parental amino acid sequence of the protein to be mutated and deriving therefrom a parental nucleotide sequence encoding the amino acid sequence, optionally selecting sites within this parental amino acid sequence, mutation of which is more desirable or less desirable, designing overlapping oligonucleotides encoding parts of the protein and harbouring one or more codon changes as compared to the corresponding parental nucleotide sequence, composing of the overlapping oligonucleotides a population of mutated nucleotide sequences encoding mutated versions of the parent protein thus establishing a library, and expressing the mutated versions of the mutated nucleotide sequences to obtain a population of mutated proteins.
(FR)
L'invention concerne une population de molécules protéiques ayant une distribution de mutations spécifiques dans la séquence d'acides aminés par rapport à une protéine parent. Pour obtenir cette population de molécules protéiques, on crée une séquence d'acides aminés parentale de la protéine à muter; on en dérive une séquence de nucléotides parentale codant pour la séquence d'acides aminés; on sélectionne éventuellement, à l'intérieur de cette séquence d'acides aminés parentale, des sites dont la mutation est plus ou moins désirable; on met au point des oligonucléotides qui se chevauchent, codant pour certaines parties de la protéine et hébergeant une ou plusieurs modifications des codons par rapport à la séquence de nucléotides parentale correspondante; on compose à partir des oligonucléotides chevauchants une population de séquences de nucléotides mutées codant pour des versions mutées de la protéine parent, ce qui permet de créer une bibliothèque; et on exprime les versions mutées des séquences de nucléotides mutées, de façon à obtenir une population de protéines mutées.
Also published as
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