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1. WO1997029127 - RECOMBINANT PREPARATION OF CALCITONIN FRAGMENTS AND USE THEREOF IN THE PREPARATION OF CALCITONIN AND RELATED ANALOGS

Publication Number WO/1997/029127
Publication Date 14.08.1997
International Application No. PCT/US1997/001652
International Filing Date 04.02.1997
Chapter 2 Demand Filed 13.08.1997
IPC
C07K 14/585 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
575Hormones
585Calcitonins
C12N 9/88 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88Lyases (4.)
CPC
C07K 14/585
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
575Hormones
585Calcitonins
C07K 2319/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
C12N 9/88
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
88Lyases (4.)
Applicants
  • BIONEBRASKA, INC. [US]/[US]
Inventors
  • WAGNER, Fred, W.
  • STOUT, Jay, S.
  • HENRIKSEN, Dennis, B.
  • PARTRIDGE, Bruce, E.
  • HOLMQUIST, Bart
  • FRANK, Julie, A.
Agents
  • BRUESS, Steven, C.
Priority Data
08/595,86806.02.1996US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) RECOMBINANT PREPARATION OF CALCITONIN FRAGMENTS AND USE THEREOF IN THE PREPARATION OF CALCITONIN AND RELATED ANALOGS
(FR) PREPARATION PAR RECOMBINAISON DE FRAGMENTS DE CALCITONINE ET UTILISATION DE CEUX-CI POUR LA PREPARATION DE CALCITONINE ET D'ANALOGUES APPARENTES
Abstract
(EN)
A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.
(FR)
L'invention concerne un procédé de préparation par recombinaison d'un fragment de calcitonine et l'utilisation dudit fragment pour la préparation de calcitonine et d'analogues apparentés. Selon le procédé, on produit par recombinaison une protéine de fusion qui contient le fragment de calcitonine lié à une anhydrase carbonique. Puis on sépare la protéine de fusion produite par recombinaison, de façon à produire un polypepetide contenant le fragment de calcitonine. L'invention concerne également un procédé permettant de produire un carba-analogue de la calcitonine, selon lequel on condense un désamino-nonapeptide avec le fragment de calcitonine produit par recombinaison.
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