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1. WO1997028252 - PROCESS FOR AGGREGATING CELLS AND FORMING SHEETS OF MAMMALIAN TISSUE IN A HORIZONTALLY ROTATING BIOREACTOR

Publication Number WO/1997/028252
Publication Date 07.08.1997
International Application No. PCT/US1997/001732
International Filing Date 30.01.1997
Chapter 2 Demand Filed 21.08.1997
IPC
C12N 5/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C12N 5/071 2010.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
CPC
C12N 5/0062
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0062General methods for three-dimensional culture
C12N 5/0677
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0676Pancreatic cells
0677Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
Applicants
  • VIVORX PHARMACEUTICALS, INC. [US]/[US] (AllExceptUS)
  • SPAULDING, Glenn, F. [US]/[US] (UsOnly)
Inventors
  • SPAULDING, Glenn, F.
Agents
  • REITER, Stephen, E.
Priority Data
60/010,87931.01.1996US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PROCESS FOR AGGREGATING CELLS AND FORMING SHEETS OF MAMMALIAN TISSUE IN A HORIZONTALLY ROTATING BIOREACTOR
(FR) PROCEDE PERMETTANT D'AGREGER DES CELLULES ET DE FORMER DES FEUILLES DE TISSU DE MAMMIFERE DANS UN BIOREACTEUR A ROTATION HORIZONTALE
Abstract
(EN)
A novel process is disclosed for aggregating mammalian cells and for forming sheets of mammalian cells employing a horizontally rotating bioreactor. The process is a single step process that is more efficient and effective than conventional approaches, yet allows for 3-dimensional cellular growth to achieve cell aggregates over 1 cm in size. The invention process is useful, for example, in developing tissues for transplantation. In accordance with another aspect of the invention, compositions and methods are provided for growth and proliferation of living cells using the novel combination of cell de-differentiation, followed by cell aggregation, followed by differentiation, culminating in proliferation of the original organ or cell type. De-differentiation brings particular cell types back to a less differentiated cellular state in which growth is not blocked, thus making the cells more amenable to rapid proliferation to obtain very large volumes. Cells may be de-differentiated in vessels with either adherent or non-adherent surfaces, including T-flasks, bags, multiwell plates, and petri dishes. Many types of cells may be de-differentiated for rapid proliferation (e.g., pancreatic islet cells, pancreatic duct cells, pancreatic beta cells, pancreatic acinar cells, hepatocytes, cartilage cells (chondrocytes), epithelial cells, as well as other cell types that are known in the art).
(FR)
Procédé nouveau permettant d'agréger des cellules de mammifère et de former des feuilles de cellules de mammifère au moyen d'un bioréacteur à rotation horizontale. Il s'agit d'un procédé en une seule étape plus rentable et plus efficace que les méthodes classiques, permettant cependant une croissance cellulaire tridimensionnelle donnant des agrégats de cellules de plus de 1 cm de dimension. Le procédé décrit est utile, par exemple, au développement des tissus pour la transplantation. Selon un autre aspect de l'invention, on décrit des compositions et des méthodes permettant la croissance et la prolifération des cellules vivantes au moyen d'un enchaînement original de dédifférenciation des cellules, suivie d'agrégation de cellule, suivie de différenciation, pour aboutir à la prolifération du type d'organe ou de cellule original. La dédifférenciation ramène certains types de cellules particuliers à un état cellulaire moins différencié, dans lequel la croissance n'est pas bloquée, ce qui rend les cellules plus sujettes à la prolifération rapide si l'on souhaite obtenir des volumes très importants. Les cellules peuvent être dédifférenciées dans des récipients présentant soit une surface adhérente, soit une surface non adhérente, tels que flacons en T, sacs, plateaux multi-puits et boîtes de Petri. De nombreux types de cellules peuvent être dédifférenciées pour une prolifération rapide, par exemple, cellules d'îlot pancréatique, cellules de conduit pancréatique, cellules bêta pancréatiques, cellules acineuses pancréatiques, hépatocytes, cellules de cartilage (chondrocytes), cellules épithéliales, ainsi que d'autres types de cellules connues dans cette technique.
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