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Machine translation
1. (WO1997020078) METHODS FOR GENERATING POLYNUCLEOTIDES HAVING DESIRED CHARACTERISTICS BY ITERATIVE SELECTION AND RECOMBINATION
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/1997/020078    International Application No.:    PCT/US1996/019256
Publication Date: 05.06.1997 International Filing Date: 02.12.1996
Chapter 2 Demand Filed:    26.06.1997    
IPC:
C07K 14/435 (2006.01), C07K 14/545 (2006.01), C12N 9/38 (2006.01), C12N 9/86 (2006.01), C12N 15/10 (2006.01), C12N 15/52 (2006.01), C12N 15/64 (2006.01)
Applicants: MAXYGEN, INC. [US/US]; 3410 Central Expressway, Santa Clara, CA 95051 (US) (For All Designated States Except US).
STEMMER, Willem, P., C. [NL/US]; (US) (For US Only).
CRAMERI, Andreas [CH/US]; (US) (For US Only)
Inventors: STEMMER, Willem, P., C.; (US).
CRAMERI, Andreas; (US)
Agent: LIEBESCHUETZ, Joe; Townsend and Townsend and Crew L.L.P., 8th floor, Two Embarcadero Center, San Francisco, CA 94111-3834 (US)
Priority Data:
08/564,955 30.11.1995 US
08/621,859 25.03.1996 US
Title (EN) METHODS FOR GENERATING POLYNUCLEOTIDES HAVING DESIRED CHARACTERISTICS BY ITERATIVE SELECTION AND RECOMBINATION
(FR) PROCEDE D'ELABORATION DE POLYNUCLEOTIDES PRESENTANT DES CARACTERISTIQUES DESIREES PAR SELECTION ITERATIVE ET RECOMBINAISON
Abstract: front page image
(EN)A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by $i(in vitro) or $i(in vivo) recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution $i(in vitro) or $i(in vivo) of proteins.
(FR)L'invention porte sur une méthode de réassemblage d'ADN après sa fragmentation aléatoire et son application à la mutagenèse de séquences d'acide nucléique par recombinaison $i(in vitro) ou $i(in vivo). Elle porte en particulier sur une méthode d'obtention de fragments d'acide nucléique ou de polynucléotides codant pour des protéines mutantes. Elle porte en outre sur une méthode de répétition de cycles de mutagenèse, de brassage et de sélection permettant une évolution dirigée $i(in vitro) ou $i(in vivo) des protéines.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, US, UZ, VN.
African Regional Intellectual Property Organization (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)