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1. (WO1997019351) METHOD OF PREPARING CELL-CONTAINING BRONCHIAL SECRETIONS FOR CYTOLOGICAL ANALYSIS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/019351 International Application No.: PCT/EP1996/004856
Publication Date: 29.05.1997 International Filing Date: 06.11.1996
Chapter 2 Demand Filed: 20.06.1997
IPC:
C12Q 1/24 (2006.01) ,G01N 1/30 (2006.01) ,G01N 33/50 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
24
Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact micro-organism
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
30
Staining; Impregnating
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Applicants:
BÖCKING, Alfred [DE/DE]; DE
BERG, Alexander [DE/DE]; DE
Inventors:
BÖCKING, Alfred; DE
BERG, Alexander; DE
Agent:
GLAWE, DELFS, MOLL & PARTNER; Liebherrstrasse 20 D-80538 München, DE
Priority Data:
195 43 252.520.11.1995DE
Title (DE) VERFAHREN ZUM VORBEREITEN VON ZELLHALTIGEN BRONCHIALSEKRETEN FÜR EINE ZYTOLOGISCHE DIAGNOSTIK
(EN) METHOD OF PREPARING CELL-CONTAINING BRONCHIAL SECRETIONS FOR CYTOLOGICAL ANALYSIS
(FR) PROCEDE POUR PREPARER DES SECRETIONS BRONCHIQUES CONTENANT DES CELLULES POUR UN DIAGNOSTIC CYTOLOGIQUE
Abstract:
(DE) Erfindungsgemäß wird ein Verfahren zum Vorbereiten von zellhaltigen Bronchialsekreten und/oder Sputum für eine zytologische Diagnostik geschaffen, das die folgenden Schritte aufweist: a) Zugabe eines Mukolytikums und chemische Mukolyse des Schleims; b) differentielles Zentrifugieren in einem flüssigen Medium, dessen Dichte zwischen der Dichte der Zellen in dem Bronchialsekret bzw. dem Sputum und der Dichte des Schleims liegt; c) Abtrennen der sedimentierten Zellen vom schleimhaltigen Zentrifugat. Durch das erfindungsgemäße Verfahren ist eine im wesentlichen vollständige Abtrennung der zu diagnostizierenden Zellen von störenden Schleimbestandteilen möglich.
(EN) The invention concerns a method of preparing cell-containing bronchial secretions and/or sputum for cytological analysis, the method comprising the following steps: a) addition of a mucolytic and chemical mucolysis of the mucus, b) differential centrifugation of the mucus in a liquid whose density lies between that of the cells in the secretion or sputum and that of the mucus, c) separation of the sedimented cells from the centrifugate containing the mucus. The method proposed enables the cells to be analysed to be separated essentially completely from interfering mucus constituents.
(FR) L'invention concerne un procédé pour préparer des sécrétions bronchiques et/ou expectorations contenant des cellules afin de réaliser un diagnostic cytologique. Ce diagnostic comporte les étapes suivantes: a) addition d'un mucolytique et mucolyse chimique du mucus, b) centrifugation différentielle dans un liquide dont la densité se situe entre la densité des cellules contenues dans les sécrétions bronchiques ou les expectorations et la densité du mucus, c) séparation des cellules sédimentées du centrifugat contenant le mucus. Le procédé de l'invention permet de séparer sensiblement complètement les cellules à examiner des composants muqueux perturbateurs.
Designated States: AT, AU, BB, BG, BR, BY, CA, CH, CN, CZ, DE, DK, ES, FI, GB, HU, IL, JP, KP, KR, KZ, LK, LU, LV, MG, MN, MW, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SK, UA, US, UZ, VN
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: German (DE)
Filing Language: German (DE)
Also published as:
AU1996075666