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1. (WO1997019103) NOVEL SYNTHETIC SUBSTRATE FOR ACTIVITY ASSAY HAVING CHROMOPHORE OR FLUOROCHROMOPHORE ACTIVE AGAINST HEPATITIS C VIRUS NS3 PROTEASE
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/019103 International Application No.: PCT/JP1996/003398
Publication Date: 29.05.1997 International Filing Date: 20.11.1996
Chapter 2 Demand Filed: 20.01.1997
IPC:
C07K 14/18 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005
from viruses
08
RNA viruses
18
Togaviridae, e.g. flavivirus, pestivirus, yellow fever virus, hepatitis C virus, japanese encephalitis virus
Applicants:
RATIONAL DRUG DESIGN LABORATORIES [JP/JP]; 1-1, Misato 4-chome Matsukawa-machi Fukushima-shi Fukushima 960-12, JP (AllExceptUS)
SHIMIZU, Yasuaki [JP/JP]; JP (UsOnly)
YAMAJI, Kayo [JP/JP]; JP (UsOnly)
MASUHO, Yasuhiko [JP/JP]; JP (UsOnly)
SHIMOTOHNO, Kunitada [JP/JP]; JP (UsOnly)
Inventors:
SHIMIZU, Yasuaki; JP
YAMAJI, Kayo; JP
MASUHO, Yasuhiko; JP
SHIMOTOHNO, Kunitada; JP
Agent:
SHIMIZU, Hatsushi; Kantetsu Tsukuba Building 6F 1-1-1, Oroshi-machi Tsuchiura-shi Ibaraki 300, JP
Priority Data:
7/30488122.11.1995JP
Title (EN) NOVEL SYNTHETIC SUBSTRATE FOR ACTIVITY ASSAY HAVING CHROMOPHORE OR FLUOROCHROMOPHORE ACTIVE AGAINST HEPATITIS C VIRUS NS3 PROTEASE
(FR) NOUVEAU SUBSTRAT SYNTHETIQUE POUR L'ESSAI D'ACTIVITE DE CHROMOPHORES OU DE FLUOROCHROMOPHORES CONTRE LA PROTEASE NS3 DU VIRUS DE L'HEPATITE C
Abstract:
(EN) A synthetic substrate which contains a specific amino acid sequence, has a fluorochromophore or chromophore covalently bonded to the C-terminus, and carries at least one amino acid inhibiting the aminopeptidase digestion on the N-terminal side of the above sequence. The use of this substrate makes it possible to efficiently assay the activity of an NS3 protease. By effecting the assay in the presence of NS4A, the detection sensitivity can be further elevated.
(FR) L'invention concerne un substrat synthétique contenant une séquence d'acides aminés spécifiques ayant un fluorochromophore ou un chromophore à liaison covalente avec l'extrémité C, et comportant au moins un acide aminé inhibant la digestion d'aminopeptidase du côté de l'extrémité N de la séquence considérée. Grâce à ce substrat, on peut tester efficacement l'activité de protéases NS3. Il est possible d'accroître encore la sensibilité de détection si l'on conduit l'essai en présence de NS4A.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IL, IS, JP, KE, KG, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, US, UZ, VN
African Regional Intellectual Property Organization (ARIPO) (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
AU1996075889