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1. (WO1997018834) THERAPEUTIC FRAGMENTS OF VON WILLEBRAND FACTOR
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/018834 International Application No.: PCT/US1996/009013
Publication Date: 29.05.1997 International Filing Date: 06.06.1996
Chapter 2 Demand Filed: 18.12.1996
IPC:
A61K 38/00 (2006.01) ,C07K 14/755 (2006.01)
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38
Medicinal preparations containing peptides
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
745
Blood coagulation or fibrinolysis factors
755
Factors VIII
Applicants:
CENTEON L.L.C. [US/US]; 1020 First Avenue King of Prussia, PA 19406, US (AllExceptUS)
FARB, David, L. [US/US]; US (UsOnly)
HRINDA, Michael, E. [US/US]; US (UsOnly)
LEE, Ted, C., K. [US/US]; US (UsOnly)
PRIOR, Christopher, P. [GB/US]; US (UsOnly)
WEBER, David [US/US]; US (UsOnly)
Inventors:
FARB, David, L.; US
HRINDA, Michael, E.; US
LEE, Ted, C., K.; US
PRIOR, Christopher, P.; US
WEBER, David; US
Agent:
BARRON, Alexis ; Synnestvedt & Lechner 2600 Aramark Tower 1101 Market Street Philadelphia, PA 19107-2950, US
Priority Data:
08/487,44507.06.1995US
Title (EN) THERAPEUTIC FRAGMENTS OF VON WILLEBRAND FACTOR
(FR) FRAGMENTS THERAPEUTIQUES DU FACTEUR VON WILLEBRAND
Abstract:
(EN) Processes for preparing aqueous solutions of cysteine-altered von Willebrand Factor fragment which are substantially free of aggregate and capable of therapeutic use for treating thrombosis are provided. The claimed process comprises providing an aqueous solution of vWF fragment and denaturant and containing undesired contaminants, said solution having an acidic pH; separating said contaminants from said solution by contacting said solution with an affinity chromatography medium to which said vWF fragments adhere; eluting said vWF fragment from said affinity chromatography medium in the presence of the denaturant; and separating the eluted fragment from said denaturant while maintaining the aqueous solution of the fragment at a pH of about 2.5 to less than abouth 5.5 to increase monomerization of, and decrease aggregation of, said fragment, thereby forming an aqueous solution of vWF fragment which is substantially free of aggregate.
(FR) La présente invention concerne des procédés de préparation de solutions de fragments du facteur von Willebrand modifiés par la cystéine, sensiblement exempts d'agrégats et pouvent être employés en thérapeutique pour traiter la thrombose. Le procédé selon l'invention comprend la fourniture d'une solution aqueuse de fragments de FvW et d'un dénaturant contenant des impuretés indésirables, cette solution ayant un pH acide, la séparation des impuretés et de la solution par sa mise en contact avec un produit de chromatographie par affinité auquel les fragments de FvW adhèrent, l'élution desdits fragments de ce produit en présence du dénaturant et la séparation des fragments élués et du dénaturant, le pH de la solution aqueuse de fragments étant maintenu à une valeur d'environ 2,5 à moins d'environ 5,5 pour accroître la monomérisation de ces fragments et réduire leur agrégation, ce qui donne une solution aqueuse de fragments de FvW qui est sensiblement exempte d'agrégats.
Designated States: AL, AM, AT, AU, AZ, BB, BG, BR, BY, CA, CH, CN, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IL, IS, JP, KE, KG, KP, KR, KZ, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, US, UZ, VN
African Regional Intellectual Property Organization (ARIPO) (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
MXPA/a/1997/009893EP0833659JP2001517197 CA2223702KR1019990022634AU1996060471