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1. (WO1997018566) METHOD FOR THE DETECTION OF CELLULAR ABNORMALITIES USING FOURIER TRANSFORM INFRARED SPECTROSCOPY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/018566 International Application No.: PCT/US1996/018116
Publication Date: 22.05.1997 International Filing Date: 12.11.1996
Chapter 2 Demand Filed: 04.06.1997
IPC:
G01J 3/28 (2006.01) ,G01J 3/453 (2006.01) ,G01N 21/35 (2006.01)
G PHYSICS
01
MEASURING; TESTING
J
MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
3
Spectrometry; Spectrophotometry; Monochromators; Measuring colours
28
Investigating the spectrum
G PHYSICS
01
MEASURING; TESTING
J
MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
3
Spectrometry; Spectrophotometry; Monochromators; Measuring colours
28
Investigating the spectrum
45
Interferometric spectrometry
453
by correlation of the amplitudes
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
17
Systems in which incident light is modified in accordance with the properties of the material investigated
25
Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
31
Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
35
using infra-red light
Applicants:
BIO-RAD LABORATORIES, INC. [US/US]; 1000 Alfred Nobel Drive Hercules, CA 94547, US (AllExceptUS)
COHENFORD, Menashi, A. [US/US]; US (UsOnly)
BHANDARE, Prashant, S. [IN/US]; US (UsOnly)
CAHN, Frederick [US/US]; US (UsOnly)
KRISHNAN, Krishnaswamy [US/US]; US (UsOnly)
RIGAS, Basil [US/US]; US (UsOnly)
Inventors:
COHENFORD, Menashi, A.; US
BHANDARE, Prashant, S.; US
CAHN, Frederick; US
KRISHNAN, Krishnaswamy; US
RIGAS, Basil; US
Agent:
KEZER, William, B. ; Townsend and Townsend and Crew L.L.P. Two Embarcadero Center, 8th floor San Francisco, CA 94111, US
Priority Data:
08/558,13013.11.1995US
Title (EN) METHOD FOR THE DETECTION OF CELLULAR ABNORMALITIES USING FOURIER TRANSFORM INFRARED SPECTROSCOPY
(FR) PROCEDE POUR DETECTER DES ANOMALIES CELLULAIRES EN UTILISANT LA SPECTROSCOPIE INFRAROUGE A TRANSFORMEE DE FOURIER
Abstract:
(EN) This invention teaches a method to identify cellular abnormalities which are associated with disease states. In one aspect, the invention is a method to distinguish premalignant and malignant stages of cervical cancer from normal cervical cells. The method utilizes infrared (IR) spectra of exfoliated cervical cells which are dried on an infrared transparent matrix and scanned at the frequency range from 3000-950 cm-1. The identification of samples is based on establishing a calibration using a representative set of spectra of normal, dysplastic and malignant specimens. During the calibration process, multivariate techniques such as Principal Component Analysis (PCA) and/or Partial Least Squares (PLS) are used. PCA and PLS reduce the data based on maximum variations between the spectra, and generate clusters in a multidimensional space representing the different populations. The utilization of Mahalinobis distances, or linear regression (e.g., Principle Component Regression on the reduced data from PCA) form the basis for the discrimination. This method is simple to use and achieves statistically reliable distinction between the following groups of cervical smears: normal (individuals with no prior history of dysplasia), dysplasia and malignant samples. Further, this invention discloses a method to obtain the IR spectrum of individual cervical cells fixed on an infrared transparent matrix and to use the spectra of the individual cells in the method described above. In an additional aspect, the invention is a method for using vibrational spectroscopic imaging to distinguish between normal and diseased cells.
(FR) Cette invention concerne un procédé pour identifier des anomalies cellulaires associées à des états pathologiques. Dans l'un de ses aspects, cette invention se rapporte à un procédé permettant de distinguer entre des états prémalins et malins de cellules cervicales cancéreuses et des états normaux de ces cellules cervicales. Ledit procédé utilise des spectres à infrarouge (IR) de cellules cervicales exfoliées, qui sont séchées sur une matrice transparente infrarouge et balayées à une fréquence comprise entre 3000 et 950 cm-1. L'identification des échantillons est basée sur l'établissement d'un étalonnage utilisant un groupe représentatif de spectres de spécimens normaux, dysplasiques et malins. Pendant ce procédé d'étalonnage, on utilise des techniques d'analyse multivariées, telles que l'analyse en composantes principales (PCA) et/ou l'analyse de régression partielle par les moindres carrés (PLS). Ces techniques PCA et PLS réduisent les données basées sur des variations maximales entre les spectres et génèrent des grappes dans un espace multidimensionnel représentant les différentes populations. L'utilisation des distances de Mahalinobis ou de la régression linéaire (par exemple la régression en composantes principales sur les données réduites provenant de la technique PCA) forme la base pour parvenir à établir une discrimination entre ces cellules. Ce procédé est simple à utiliser et permet d'effectuer une distinction statistiquement fiable entre les groupes suivants de frottis cervicaux: échantillons normaux (provenant d'individus n'ayant pas d'antécédent de dysplasie), échantillons dysplasiques et échantillons malins. Cette invention se rapporte en outre à un procédé permettant d'obtenir le spectre IR de cellules cervicales individuelles fixées sur une matrice transparente infrarouge et d'utiliser les spectres de ces cellules individuelles dans le procédé décrit ci-dessus. Dans l'un de ses autres aspects, cette invention concerne un procédé utilisant l'imagerie spectroscopique vibrationnelle pour établir une distinction entre des cellules normales et des cellules malades.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, US, UZ, VN
African Regional Intellectual Property Organization (ARIPO) (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0873506JPH11502935 AU1996077281