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1. (WO1997018474) METHOD OF SELECTING A POPULATION OR SUBPOPULATION OF A SAMPLE UTILIZING PARTICLE AND GRAVITY SEDIMENTATION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/018474 International Application No.: PCT/US1996/017577
Publication Date: 22.05.1997 International Filing Date: 05.11.1996
Chapter 2 Demand Filed: 13.06.1997
IPC:
G01N 33/537 (2006.01) ,G01N 33/569 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
536
with immune complex formed in liquid phase
537
with separation of immune complex from unbound antigen or antibody
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
569
for micro-organisms, e.g. protozoa, bacteria, viruses
Applicants:
COULTER INTERNATIONAL CORP. [US/US]; Mail Code 32-A02 11800 S.W. 147 Avenue Miami, FL 33196, US
Inventors:
COULTER, Wallace, H.; US
ZWERNER, Robert, K.; US
SCHMITTLING, Robert, J.; US
RUSSELL, Thomas, R.; US
Agent:
NAKAMURA, Jackie, N.; Cooley Godward L.L.P. Five Palo Alto Square 3000 El Camino Real Palo Alto, CA 94306-2155, US
Priority Data:
08/556,66713.11.1995US
Title (EN) METHOD OF SELECTING A POPULATION OR SUBPOPULATION OF A SAMPLE UTILIZING PARTICLE AND GRAVITY SEDIMENTATION
(FR) PROCEDE DE SELECTION D'UNE POPULATION OU D'UNE SOUS-POPULATION DANS UN ECHANTILLON A L'AIDE DE PARTICULES ET DE LA SEDIMENTATION GRAVIMETRIQUE
Abstract:
(EN) A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles (14) have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample (12) by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including an enriched population is separated from the particles with the selected population bound thereto. The enriched populations in the biological sample supernantant can be further enriched by multiple removal steps.
(FR) Procédé de séparation permettant de séparer une population désirée ou indésirable d'un échantillon biologique à l'aide de particules denses relativement lourdes et de la sédimentation gravimétrique. Les particules (14) portent fixés sur elles un ou plusieurs réactants spécifiques de la population sélectionnée, qui vont se fixer sur celle-ci. De préférence, on mélange les particules à l'échantillon (12) par sédimentation répétée dans une partie substantielle de l'échantillon, de façon à les fixer sur la population sélectionnée. Puis on laisse les particules fixées à la population sélectionnée se déposer de façon préférentielle dans l'échantillon, et on sépare des particules portant fixées sur elles la population sélectionnée le surnageant contenant une population enrichie. On peut ensuite, par de multiples étapes supplémentaires, continuer à enrichir les populations déjà enrichies contenues dans le surnageant biologique.
Designated States: CA, JP
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0874991JP2000500388 CA2237119