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1. (WO1997018471) USE OF NUCLEAR MAGNETIC RESONANCE TO IDENTIFY LIGANDS TO TARGET BIOMOLECULES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/018471 International Application No.: PCT/US1996/018270
Publication Date: 22.05.1997 International Filing Date: 13.11.1996
Chapter 2 Demand Filed: 01.05.1997
IPC:
G01N 24/08 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/542 (2006.01) ,G01N 33/68 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
24
Investigating or analysing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
08
by using nuclear magnetic resonance
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
536
with immune complex formed in liquid phase
542
with steric inhibition or signal modification, e.g. fluorescent quenching
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68
involving proteins, peptides or amino acids
Applicants:
ABBOTT LABORATORIES [US/US]; CHAD 0377/AP6D-2 100 Abbott Park Road Abbott Park, IL 60064-3500, US
Inventors:
FESIK, Stephen, W.; US
HAJDUK, Philip, J.; US
Agent:
BROWDER, Monte, R. ; Abbott Laboratories CHAD 0377/AP6D-2 100 Abbott Park Road Abbott Park, IL 60064-3500, US
BACH, Mark, C.; Abbott Laboratories CHAD 0377/AP6D-2 100 Abbott Park Road Abbott Park, IL 60064-3500, US
Priority Data:
08/555,69114.11.1995US
Title (EN) USE OF NUCLEAR MAGNETIC RESONANCE TO IDENTIFY LIGANDS TO TARGET BIOMOLECULES
(FR) EMPLOI DE LA RESISTANCE MAGNETIQUE NUCLEAIRE POUR IDENTIFIER DES LIGANDS A DES BIOMOLECULES CIBLES
Abstract:
(EN) The present invention provides a process for identifying compounds which bind to a specific target molecule. The process includes the steps of: a) generating a first two-dimensional 15N/1H NMR correlation spectrum of a 15N-labeled target molecule; b) exposing the labeled target molecule to one or a mixture of chemical compounds; c) generating a second two-dimensional 15N/1H NMR correlation spectrum of the labeled target molecule that has been exposed to one or a mixture of compounds in step (b); and d) comparing said first and second two-dimensional 15N/1H NMR correlation spectra to determine differences between said first and said second spectra, the differences indentifying the presence of one or more compounds that are ligands which have bound to the target molecule.
(FR) La présente invention concerne un procédé pour identifier des composés se liant à une molécule cible spécifique. Ce procédé consiste à: (a) créer un premier spectre de corrélation bidimensionnel 15N/1H NMR d'une molécule cible marquée 15N; b) exposer la molécule cible marquée à un composé ou à un mélange de composés chimiques; c) créer un deuxième spectre de corrélation bidimensionnel 15N/1H NMR de la molécule cible marquée qui a été exposée à un composé ou à un mélange de composés lors de l'étape (b); et d) comparer lesdits premier et deuxième spectres de corrélation bidimensionnels 15N/1H NMR en vue de déterminer les différences entre ces premier et deuxième spectres, ces différences identifiant la présence d'un ou de plusieurs composés qui sont des ligands s'étant liés à la molécule cible.
Designated States: AU, CA, IL, JP, MX
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
MXPA/a/1998/003810IL123923IL155988EP0866967EP0981049JPH11513498
CA2237343DK0866967AU1996077328