Search International and National Patent Collections
Some content of this application is unavailable at the moment.
If this situation persists, please contact us atFeedback&Contact
1. (WO1997018298) EX VIVO CULTURE OF STEM CELLS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/018298 International Application No.: PCT/US1996/018222
Publication Date: 22.05.1997 International Filing Date: 13.11.1996
IPC:
C12N 5/0783 (2010.01) ,C12N 5/0789 (2010.01) ,A61K 35/12 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
078
Cells from blood or from the immune system
0783
T cells; NK cells; Progenitors of T or NK cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
078
Cells from blood or from the immune system
0789
Stem cells; Multipotent progenitor cells
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
Applicants:
REGENTS OF THE UNIVERSITY OF MINNESOTA [US/US]; Morrill Hall 100 Church Street S.E. Minneapolis, MN 55455, US (AllExceptUS)
VERFAILLIE, Catherine, M. [BE/US]; US (UsOnly)
MCGLAVE, Philip, B. [US/US]; US (UsOnly)
MILLER, Jeffrey, S. [US/US]; US (UsOnly)
Inventors:
VERFAILLIE, Catherine, M.; US
MCGLAVE, Philip, B.; US
MILLER, Jeffrey, S.; US
Agent:
VIKSNINS, Ann, S.; Schwegman, Lundberg, Woessner & Kluth P.O. Box 2938 Minneapolis, MN 55402, US
Priority Data:
60/006,73714.11.1995US
Title (EN) EX VIVO CULTURE OF STEM CELLS
(FR) CULTURE EX VIVO DE CELLULES SOUCHES
Abstract:
(EN) A method for the culture of hematopoietic cells is provided comprising culturing said cells in stromal conditioned medium comprising one or more cytokines selected from the group consisting of MIP-1$g(a), IL-3, SCF, BB10010 and PF-4, so that the ability of the stem cells to self-replicate and differentiate is maintained or the ability of the committed progenitors to expand and differentiate is maintained.
(FR) La présente invention concerne un procédé de culture de cellules hématopoïétiques. Il comprend la culture de ces cellules dans un milieu conditionné par le stroma et comportant une ou plusieurs cytokines choisies dans le groupe que constituent MIP-$g(a), IL-3, SCF, BB10010 et PF-4, de telle sorte que la capacité des cellules souches à s'autorépliquer et à se différencier est maintenue, ou la capacité des ascendants impliqués à l'expansion et à la différenciation est maintenue.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IL, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, US, UZ, VN
African Regional Intellectual Property Organization (ARIPO) (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
US5922597AU1997011193