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1. (WO1997018233) METHOD FOR PRODUCING A CORRECTLY FOLDED, BIOLOGICAL ACTIVE RECOMBINANT PROTEIN
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/018233 International Application No.: PCT/SE1996/001456
Publication Date: 22.05.1997 International Filing Date: 12.11.1996
Chapter 2 Demand Filed: 12.06.1997
IPC:
C07K 1/113 (2006.01) ,C07K 14/61 (2006.01) ,C07K 14/65 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
107
by chemical modification of precursor peptides
113
without change of the primary structure
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
575
Hormones
61
Growth hormone (GH) (Somatotropin)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
575
Hormones
65
Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
Applicants:
PHARMACIA & UPJOHN AB [SE/SE]; S-112 87 Stockholm, SE (AllExceptUS)
GUSTAFSSON, Jan-Gunnar [SE/SE]; SE (UsOnly)
ÖHMAN, Johan [SE/SE]; SE (UsOnly)
Inventors:
GUSTAFSSON, Jan-Gunnar; SE
ÖHMAN, Johan; SE
Agent:
TANNERFELDT, Agneta ; Pharmacia & Upjohn AB S-112 87 Stockholm, SE
Priority Data:
9504019-213.11.1995SE
Title (EN) METHOD FOR PRODUCING A CORRECTLY FOLDED, BIOLOGICAL ACTIVE RECOMBINANT PROTEIN
(FR) PROCEDE DE PRODUCTION D'UNE PROTEINE DE RECOMBINAISON ACTIVE, BIOLOGIQUE, CORRECTEMENT REPLIEE
Abstract:
(EN) The present invention relates to a method for producing a correctly folded, biological active recombinant protein or polypeptide, comprising the steps of expression of the protein in prokaryotic cells, harvest of the cells, direct solubilization of the cells in a buffer at pH about 8 to 11 and thereafter dilution with water and a diluent. The protein or polypeptide is preferably GH, IGF-I or IGF-II.
(FR) La présente invention se rapporte à un procédé de production d'une protéine ou d'un polypeptide de recombinaison, actif, biologique et correctement replié, ce procédé consistant à exprimer la protéine dans des cellules procaryotiques, prélever les cellules, les solubiliser directement dans un tampon à un pH compris entre environ 8 et 11, puis les diluer avec de l'eau et un diluant. La protéine ou le polypeptide est de préférence GH, IGF-I ou IGF-II.
Designated States: AU, CA, JP, NO, US
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
NO19982155EP0952981US6605706JP2000500023 CA2236751DE000069631906
AU1996076596