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1. (WO1997017613) PEPTIDES WHICH ENHANCE TRANSPORT ACROSS TISSUES AND METHODS OF IDENTIFYING AND USING THE SAME
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/017613 International Application No.: PCT/IE1996/000072
Publication Date: 15.05.1997 International Filing Date: 11.11.1996
Chapter 2 Demand Filed: 21.05.1997
IPC:
C07K 14/47 (2006.01) ,C07K 14/705 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/569 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
46
from vertebrates
47
from mammals
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
569
for micro-organisms, e.g. protozoa, bacteria, viruses
Applicants:
ELAN CORPORATION, PLC [IE/IE]; Monksland Industrial Estate Athlone County Westmeath, IE (AllExceptUS)
O'MAHONY, Daniel, Joseph [IE/IE]; IE (UsOnly)
Inventors:
O'MAHONY, Daniel, Joseph; IE
Agent:
ANNE RYAN & CO.; 60 Northumberland Road Ballsbridge Dublin 4, IE
Priority Data:
60/006,46110.11.1995US
95086410.11.1995IE
Title (EN) PEPTIDES WHICH ENHANCE TRANSPORT ACROSS TISSUES AND METHODS OF IDENTIFYING AND USING THE SAME
(FR) PEPTIDES FAVORISANT LE TRANSPORT DANS LES TISSUS ET PROCEDES D'IDENTIFICATION ET D'UTILISATION CORRESPONDANTS
Abstract:
(EN) A method of identifying a peptide which permits or facilitates the transport of an active agent through a human or animal tissue. A predetermined amount of phage from a random phage library or preselected phage library is plated onto or brought into contact with a first side, preferably the apical side, of a tissue sample or polarized tissue cell culture. At a predetermined time, the phage which is transported to a second side of the tissue opposite the first side, preferably the basolateral side, is harvested to selected transported phage. This modified phage is amplified in a host. This cycle of events is repeated (using the transported phage produced in the most recent cycle) a predetermined number of times to obtain a selected phage library containing phage which can be transported from the first side to the second side. Lastly, the identity of at least one peptide coded by phage in the selected phage library is determined to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.
(FR) La présente invention concerne un procédé d'identification d'un peptide permettant ou favorisant le transport d'un principe actif au travers d'un tissu humain ou animal. Le procédé consiste à prendre une certaine quantité de phages choisis dans une bibliothèque aléatoire de phages ou une bibliothèque de phages sélectionnés, à appliquer une couche de ces phages contre un premier côté, de préférence apical, d'un échantillon tissulaire ou d'une culture polarisée de cellules tissulaires, ou à mettre en contact ces phages avec le premier côté. Le procédé consiste ensuite, à un instant défini, à récolter le phage transporté vers le second côté du tissu, à l'opposé du premier côté, de préférence le côté basolatéral, et à sélectionner le phage transporté. Le phage modifié est soumis à amplification à l'intérieur d'un hôte. Le procédé consiste alors à répéter ce cycle d'événements (en utilisant le phage transporté issu du cycle de production le plus récent) un certain nombre de fois jusqu'à obtenir une bibliothèque de phages sélectionnés contenant un phage capable d'être transporté du premier côté au second côté. Le procédé consiste enfin à déterminer, dans la bibliothèque de phages sélectionnés, l'identité de l'un au moins des peptides codés par le phage, lequel peptide permet ou favorise le transport d'un principe actif au travers d'un tissu humain ou animal.
Designated States: AL, AU, BB, BG, BR, CA, CN, CZ, EE, GE, HU, IL, IS, JP, KP, KR, LK, LT, LV, MK, MX, NO, NZ, PL, RO, RU, SG, SI, SK, TR, TT, UA, US, VN
African Regional Intellectual Property Organization (ARIPO) (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
MXPA/a/1998/003684EP0859959JP2002504072 CA2235226NZ322174AU1996075852
DK0859959