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1. (WO1997017610) METHOD FOR FLUORESCENT LABELING OF ANTIBODY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/017610 International Application No.: PCT/US1996/017826
Publication Date: 15.05.1997 International Filing Date: 05.11.1996
IPC:
G01N 33/533 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
531
Production of immunochemical test materials
532
Production of labelled immunochemicals
533
with fluorescent label
Applicants:
PHARMACEUTICAL DISCOVERY CORPORATION [US/US]; 4th floor 33 West Main Street Elmsford, NY 10523, US
Inventors:
WILSON, Bryan, R.; US
MCCABE, R., Tyler; US
RAULLI, Robert, E.; US
KEVELSON, Gary, I.; US
Agent:
PABST, Patrea, L.; Arnall Golden & Gregory 2800 One Atlantic Center 1201 West Peachtree Street Atlanta, GA 30309-3450, US
Priority Data:
555,35908.11.1995US
Title (EN) METHOD FOR FLUORESCENT LABELING OF ANTIBODY
(FR) PROCEDE DE MARQUAGE FLUORESCENT D'ANTICORPS
Abstract:
(EN) A method has been developed for labeling of antibodies with a fluorescent dye where the antibody is first bound to immobilized antigen, the antibody is labeled with the dye, the unbound dye is removed, and then the antibody is eluted from the antigen. As demonstrated by the examples, antibody immunoreactive with AI was labeled with a fluorescent dye and then used in an assay for AI. The results showed that the antibody prepared by this method had a 20 % increase in specific binding compared to radioimmunoassay (RIA).
(FR) Procédé permettant de marquer des anticorps à l'aide d'un colorant fluorescent, selon lequel ledit anticorps est d'abord lié à un antigène immobilisé, puis il est marqué avec le colorant, le colorant non lié étant éliminé, et ensuite il est élué de l'antigène. Comme le montrent les exemples, un anticorps immunoréactif avec l'angiotensine I (AI) a été marqué à l'aide d'un colorant fluorescent et ensuite utilisé dans une analyse de détection de AI. Les résultats ont montré que l'anticorps préparé selon ce procédé présente une augmentation de 20 % de la liaison spécifique par comparaison au radioimmunodosage (RAI).
Designated States: AU, CA, JP
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU1996077230