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1. (WO1997017465) MICROORGANISM QUANTITATION AND DETECTION METHOD AND KIT
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/017465 International Application No.: PCT/FR1996/001736
Publication Date: 15.05.1997 International Filing Date: 05.11.1996
Chapter 2 Demand Filed: 04.06.1997
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
MICRODIAG [FR/FR]; 24, rue de Chartres F-92200 Neuilly-sur-Seine, FR (AllExceptUS)
ANDRIEU, Jean-Marie [FR/FR]; FR (UsOnly)
LU, Wei [FR/FR]; FR (UsOnly)
Inventors:
ANDRIEU, Jean-Marie; FR
LU, Wei; FR
Agent:
LEBOYER, Jean-Jacques; Cabinet Leboyer 12, rue du Helder F-75009 Paris, FR
Priority Data:
95/1309306.11.1995FR
Title (EN) MICROORGANISM QUANTITATION AND DETECTION METHOD AND KIT
(FR) PROCEDE ET KIT POUR LA QUANTIFICATION ET LA DETECTION DES MICRO-ORGANISMES
Abstract:
(EN) A method wherein predetermined amounts of a microorganism of interest or the DNA or RNA thereof are used as the external standard; a fraction of the genome of said microorganism is extracted, revealed using a specific method or subjected to reverse transcription and/or amplification; and the DNA or RNA concentrations of the standard and target microorganisms or the target microorganism amplification products are compared with those of the external standard to determine values for the DNA or RNA concentration or the total microorganism concentration in each target microorganism sample. The method is useful for the quantitation and detection of all microorganisms.
(FR) Dans le procédé selon l'invention, on utilise en tant qu'étalon externe des quantités données du micro-organisme concerné ou de l'ADN ou ARN de ce micro-organisme; et après extraction, révélation au moyen d'une méthode spécifique ou rétrotranscription et/ou amplification d'une fraction du génome dudit micro-organisme, on effectue une comparaison des concentrations d'ADN ou d'ARN des micro-organismes étalon et cible ou des produits d'amplification des micro-organismes cible avec ceux de l'étalon externe, pour en déduire les valeurs de la concentration d'ADN ou d'ARN ou de la concentration en micro-organisme totale dans chaque échantillon de micro-organisme cible. Application à la quantification et la détection de tous les micro-organismes.
Designated States: AU, BR, CA, CZ, GE, HU, IL, IS, JP, KP, KR, LK, LR, MG, MN, MX, NO, NZ, PL, RO, SG, TR, UA, US, UZ, VN
African Regional Intellectual Property Organization (ARIPO) (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: French (FR)
Filing Language: French (FR)
Also published as:
CZPV1998-1205NO19982039EP0862654US6277560JP2000500007CN1154410
CA2236842EA199800438AU1996075004FR2740782FR2740781MYPI 96004591