Some content of this application is unavailable at the moment.
If this situation persists, please contact us atFeedback&Contact
1. (WO1997017464) USE OF O-$g0b)-D-GALACTOPYRANOSYL-D-XYLOSES FOR THE PREPARATION OF COMPOSITIONS AND SOLUTIONS INTENDED TO THE EVALUATION OF INTESTINAL LACTASE, AND PRODUCTION PROCESS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/017464 International Application No.: PCT/ES1996/000208
Publication Date: 15.05.1997 International Filing Date: 08.11.1996
IPC:
C12Q 1/34 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
34
involving hydrolase
Applicants:
CONSEJO SUPERIOR INVESTIGACTIONES CIENTIFICAS [ES/ES]; Serrano, 117 E-28006 Madrid, ES (AllExceptUS)
UNIVERSIDAD AUTONOMA [ES/ES]; Canto Blanco E-28049 Madrid, ES (AllExceptUS)
ARAGON REYES, Juan José [ES/ES]; ES (UsOnly)
CANADA VICINAY, Francisco Javier [ES/ES]; ES (UsOnly)
FERNANDEZ-MAYORALAS ALVAREZ, Alfonso [ES/ES]; ES (UsOnly)
LOPEZ ALVAREZ, Rosa [ES/ES]; ES (UsOnly)
MARTIN LOMAS, Manuel [ES/ES]; ES (UsOnly)
VILLANUEVA TORREGROZA, Daniel [CO/CO]; CO (UsOnly)
Inventors:
ARAGON REYES, Juan José; ES
CANADA VICINAY, Francisco Javier; ES
FERNANDEZ-MAYORALAS ALVAREZ, Alfonso; ES
LOPEZ ALVAREZ, Rosa; ES
MARTIN LOMAS, Manuel; ES
VILLANUEVA TORREGROZA, Daniel; CO
Agent:
OJEDA GARCIA, Pedro; Consejo Superior Investigaciones Científicas Serrano, 113 E-28006 Madrid, ES
Priority Data:
P 950218508.11.1995ES
Title (EN) USE OF O-$g0b)-D-GALACTOPYRANOSYL-D-XYLOSES FOR THE PREPARATION OF COMPOSITIONS AND SOLUTIONS INTENDED TO THE EVALUATION OF INTESTINAL LACTASE, AND PRODUCTION PROCESS
(ES) USO DE $g(b)-D-GALACTOPIRANOSIL-D-XILOSAS PARA LA PREPARACION DE COMPOSICIONES Y DISOLUCIONES DESTINADAS A LA EVALUACION DE LA LACTASA INTESTINAL, Y PROCEDIMIENTO PARA SU OBTENCION
(FR) UTILISATION DE O-$g(b)-D-GALACTOPYRANOSYL-D-XYLOSES POUR LA PREPARATION DE COMPOSITIONS ET SOLUTIONS DESTINEES A L'EVALUATION DE LA LACTASE INTESTINALE, ET PROCEDE POUR SON OBTENTION
Abstract:
(EN) There is disclosed the use of O-$g(b)-D-galactopyranosyl-D-xyloses, particularly 2-O-$g(b)-D-galactopyranosyl-D-xylose and 3-O-$g(b)-D-galactopyranosyl-D-xylose, for the preparation of compositions and solutions intended to the evaluation of the intestinal lactase, which present an affinity to the substantially enhanced lactase. There is also disclosed a process for producing O-$g(b)-D-galactopyranosyl-D-xyloses, comprising: reacting a D-xylose and a substrate of $g(b)-D-galactopyranosyl in the presence of an enzyme $g(b)-galactosidase, in an aqueous medium buffered to a pH comprised between 5.0 and 9.0 and at temperature between 4 and 37 °C; deactivating the $g(b)-galactosidase by heating at 100 °C when reaching the maximum yield of formation of disaccharides detected through thin layer chromatography, and isolating the formed disaccharides through filtration in a packed column with a thinner selected between water and water/alcohol.
(ES) Se describe el uso de O-$g(b)-D-galactopiranosil-D-xilosas, concretamente de 2-O-$g(b)-D-galactopiranosil-D-xilosa y de 3-O-$g(b)-D-galactopiranosil-D-xilosa, para la preparación de composiciones y disoluciones destinadas a la evaluación de la lactasa intestinal, que presentan una afinidad a la lactasa sustancialmente mejorada. También se describe un procedimiento para la obtención de O-$g(b)-D-galactopiranosil-D-xilosas, que comprende: hacer reaccionar una D-xilosa y un sustrato de $g(b)-D-galactopiranósido en presencia de una enzima $g(b)-galactosidasa, en un medio acuoso tamponado a pH 5,0 - 9,0 y a una temperatura entre 4 y 37 °C; desactivar la $g(b)-galactosidosa mediante calentamiento a 100 °C cuando se alcanza el máximo rendimiento de formación de disacáridos detectado mediante cromatografía en capa fina, y aislamiento de los disacáridos formados mediante filtración en columna de relleno con un diluyente seleccionado entre agua o agua/alcohol.
(FR) L'invention décrit l'utilisation de O-$g(b)-D-galactopyranosyl-D-xyloses, en particulier 2-O-$g(b)-D-galactopyranosyl-D-xylose et 3-O-$g(b)-D-galactopyranosyl-D-xylose, pour la preparation de compositions des solutions destinées à l'évaluation de la lactase intestinale, et présentant une affinité à la lactase sensiblement améliorée. On décrit aussi un procédé pour l'obtention de O-$g(b)-D-galactopyranosyl-D-xyloses, consistant à: faire réagir une D-xylose et un substrat de $g(b)-D-galactopyranosyl en présence d'une enzyme $g(b)-galactosidase, dans un milieu aqueux tamponné à un pH compris entre 5,0 et 9,0 et à une température entre 4 et 37 °C; désactiver la $g(b)-galactosidase en chauffant à 100 °C lorsqu'on atteint le rendement maximum de formation de disaccharides déetecté par chromatographie en couche fine, et isolement des disaccharides formés par filtration en colonne garnie avec un diluant sélectionné entre l'eau ou eau/alcool.
Designated States: AL, AT, AU, AZ, BB, BG, BR, BY, CA, CH, CN, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IS, JP, KE, KG, KP, KR, KZ, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, US, UZ, VN
African Regional Intellectual Property Organization (ARIPO) (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: Spanish (ES)
Filing Language: Spanish (ES)
Also published as:
CZPV1997-2527NO19973159SK100197MXPA/a/1997/005109EP0819764US5994092
JP3036848 JPH10512457 CN1177984CA2209927EA199700097KR1019980701287
NZ321607AU1996074977