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1. (WO1997017458) ACCESSORY FUNCTIONS FOR USE IN RECOMBINANT AAV VIRION PRODUCTION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/017458 International Application No.: PCT/US1996/018089
Publication Date: 15.05.1997 International Filing Date: 08.11.1996
Chapter 2 Demand Filed: 02.06.1997
IPC:
A61K 48/00 (2006.01) ,C07K 14/075 (2006.01) ,C12N 7/04 (2006.01) ,C12N 15/861 (2006.01) ,C12N 15/864 (2006.01)
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
48
Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005
from viruses
01
DNA viruses
075
Adenoviridae
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7
Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
04
Inactivation or attenuation; Producing viral sub-units
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
86
Viral vectors
861
Adenoviral vectors
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
86
Viral vectors
864
Parvoviral vectors
Applicants:
AVIGEN, INC. [US/US]; 1201 Harbor Bay Parkway #1000 Alameda, CA 94502, US
Inventors:
COLOSI, Peter, C.; US
Agent:
McCRACKEN, Thomas, P. ; Robins & Associates. Suite 200 90 Middlefield Road Menlo Park, CA 94025, US
Priority Data:
08/745,95707.11.1996US
60/006,40209.11.1995US
Title (EN) ACCESSORY FUNCTIONS FOR USE IN RECOMBINANT AAV VIRION PRODUCTION
(FR) FONCTIONS ACCESSOIRES SERVANT A PRODUIRE DES VIRIONS DE VAA RECOMBINES
Abstract:
(EN) Accessory functions capable of supporting efficient recombinant AAV (rAAV) virion production in a suitable host cell are provided. The accessory functions are in the form of one or more vectors comprising a nucleotide sequence selected from the group consisting of (i) a sequence that provides adenovirus VA RNAs, (ii) an adenovirus E4 ORF6 coding region, (iii) an adenovirus E2a 72 kD coding region, and any combination of nucleotide sequences (i), (ii) and (iii), and that are capable of being transferred between cells. Methods of producing rAAV virions are also provided. The methods can be practised to produce commercially significant levels of rAAV particles without also generating significant levels of infectious helper virus or other contaminating by-products.
(FR) On décrit des fonctions accesssoires qui permettent une production rentable de virions de virus adéno-associés (VAA) recombinés chez une cellule hôte appropriée. Elles prennent la forme d'un ou plusieurs vecteurs comprenant une séquence nucléotidique choisie dans le groupe consistant en: 1) une séquence qui donne des ARN VA d'adénovirus; 2) une région de codage E4 ORF 6 d'adénovirus; 3) une région de codage E2a de 72 dK d'adénovirus, ou l'une quelconque des combinaisons de ces séquences 1), 2) et 3), et qui peuvent être transférées entre des cellules. On décrit aussi des procédés de production de ces virions de VAA recombinés, utilisables pour produire des niveaux commercialement significatifs de particules de VAA recombinés sans qu'ils s'accompagnent de niveaux significatifs de virus auxiliaires infectieux ou d'autres sous-produits contaminants.
Designated States: CA, JP
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0862643EP1522588JP2006075171JP2009077737JP2012050453JP2001517071
CA2236968