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1. (WO1997017439) METHOD FOR EXPRESSION CLONING OF GENE ENCODING PROTEIN KINASE SUBSTRATE PROTEIN
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/017439 International Application No.: PCT/JP1996/003234
Publication Date: 15.05.1997 International Filing Date: 05.11.1996
Chapter 2 Demand Filed: 05.12.1996
IPC:
C07K 14/00 (2006.01) ,C12N 15/10 (2006.01) ,C12N 15/65 (2006.01) ,C12Q 1/48 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
65
using markers
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
48
involving transferase
Applicants:
TANAKA, Toshio [JP/JP]; JP
Inventors:
TANAKA, Toshio; JP
Agent:
ISHIDA, Takashi ; A. Aoki & Associates Toranomon 37 Mori Building 5-1, Toranomon 3-chome Minato-ku Tokyo 105, JP
Priority Data:
7/28877507.11.1995JP
Title (EN) METHOD FOR EXPRESSION CLONING OF GENE ENCODING PROTEIN KINASE SUBSTRATE PROTEIN
(FR) PROCEDE DE CLONAGE PAR EXPRESSION D'UN GENE CODANT UNE PROTEINE SERVANT DE SUBSTRAT A UNE PROTEINE KINASE
Abstract:
(EN) An efficient method for cloning a DNA which encodes a protein kinase substrate protein. The method comprises incubating on a plate a host into which a DNA inserted into an expression vector has been introduced; bringing a membrane into contact with the plate to thereby transfer the protein thus expressed from the plate onto the membrane; peeling off the membrane from the plate; adding a labeled phosphate donor and protein kinase to the membrane so as to phosphorylate the protein; detecting the phosphoric acid label bound to the protein; and then isolating the DNA from a clone on the plate corresponding to the positive site on the membrane.
(FR) Cette invention concerne un procédé efficace de clonage d'un ADN codant une protéine servant de support à une protéine kinase. Ce procédé consiste à incuber, sur une plaquette, un hôte dans lequel on a introduit un ADN qui est lui-même inséré dans un vecteur d'expression; à mettre une membrane en contact avec la plaquette de manière à transférer la protéine ainsi exprimée depuis la plaquette sur la membrane; à peler la membrane de la plaquette; à ajouter un donneur de phosphate marqué et une protéine kinase sur la membrane de manière à phosphoryler la protéine; à détecter le marqueur acide phosphorique lié à la protéine; et, enfin, à isoler l'ADN à partir d'un clone sur la plaquette correspondant au site positif de la membrane.
Designated States: AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CU, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IL, IS, KE, KG, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, US, UZ, VN
African Regional Intellectual Property Organization (ARIPO) (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
EP0863203US6010858AU1996073402