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1. (WO1997016972) PESTICIDAL COMPOSITION AND PROCESS FOR PREPARATION THEREOF
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CLAIMS

1. A pesticidal composition comprising an insecticide phase comprising one or more ingestible pesticidal components effective against insects of the order diptera in an aqueous environment and being derivable from a microorganism, the insecticide phase being dispersed in a fatty phase comprising a lipid or a mixture of lipids which is solid at ambient temperature, the composition further comprising at least one disintegrant .

2. A pesticidal composition according to claim 1 which further comprises at least one detergent.

3. A pesticidal composition according to claim 1 or 2 wherein the insects of the order diptera is selected from brachycera and nematocera, such as culicidae.

4. A composition according to any of claims 1-3 wherein the insects of the order diptera is in the larval stage.

5. A composition according to any of the preceding claims wherein the microorganism is selected from bacteria, virus or fungi .

6. A composition according to any of the preceding claims wherein the ingestible pesticidal component is derivable from bacteria of the Bacillus type, such as Bacillus sphericus and Bacillus thuringiensis, e.g. Bacillus thuringiensis var israelensis (H14) or var morrisoni or var rπedellin or from the bacterium Clostridium bifermentans .

7. A composition according to any of the proceeding claims wherein the microorganism is genetically engineered.

8. A composition according to any of the proceeding claims wherein the microorganism is genetically engineered with respect to expression and/or production of the pesticidal component resulting in increased activity or increased amount of the pesticidal component as compared to the non-engineered microorganism.

9. A composition according any of the proceeding claims wherein the microorganism express or produce the pesticidal component before or after ingestion of the composition by the insects of the order diptera.

10. A composition according to any of the proceeding claims wherein the pesticidal component comprises a toxin or mixture of toxins.

11. A composition according to any of the proceeding claims wherein the pesticidal component comprises a mixture of a chemical pesticidal component and a biological pesticidal component .

12. A composition according to any of the preceding claims wherein the insecticide phase comprises the pesticidal component in the form of a powder.

13. A composition according to any of the preceding claims wherein the pesticidal composition is in a granular form or in a powder form or mixtures thereof.

14. A composition according to any of claims 2-13 wherein the detergent is selected from ionic and non- ionic detergents or mixtures thereof .

15. A composition according to any of claims 2-14 wherein the pesticidal component comprises intact Bacillus sphericus and the detergent comprises a cationic detergent.

16. A composition according to any of the preceding claims wherein the disintegrant swells or cause the composition to increase in volume when moistened by contact with the aqueous environment.

17. A composition according to claim 16 wherein the disintegrant is selected from starches, celluloses, clays, algins, carrageenans, pectines and gums.

18. A composition according to claim 17 wherein the disinte-grant is selected from methyl cellulose, ethyl cellulose, gelatine powder, and guar gum.

19. A composition according to any of the preceding claims wherein the disintegrant is present in an amount of from about 5 to about 50 % weight/weight of the final composition, such as from about 5 to about 30 % weight/weight .

20. A composition according to any of the preceding claims further comprising a filler.

21. A composition according to claim 20 wherein the filler is selected from the group consisting of silica; alumina and carbonates such as bentonite, attapulgite, pyrophyllite, talc, kaolin, diatomaceous earth, synthetic silicas, celite, vermiculite, ground silica and sand, clay, chalk, calcium carbonate, starch, modified starch, flours and mixtures thereof.

22. A composition according to any of the preceding claims wherein air is entrapped in the composition.

23. A composition according to any of the preceding claims wherein the density of the composition is adapted so that the composition substantially floats or substantially stay in the feeding zone of the insects of the order diptera in the aqueous environment.

24. A composition according to claim 23 wherein the adaptation of the density of the composition is modified by entrap-ping air into the composition.

25. A composition according to any of the preceding claims wherein the ingestible pesticidal component of the disintegrated composition has a density adapted so that the
ingestible pesticidal component substantially stay floating in the aqueous environment .

26. A composition according to any of the preceding claims which is non-aqueous.

27. A composition according to any of the preceding claims further comprising an antioxidant.

28. A composition according to any of the proceeding claims comprising granular particles between 0.5mm and 2mm.

29. A composition according to any of the preceding claims further comprising one or more ingredients selected from UV protecting agents such as a UV- filter or a UV reflecting material and non- toxic ingredients or repellents, such as bitter components, to prevent uptake of the composition by non-target objects such as fish, birds, irammals, and non-target insects.

30. A composition according to any of claims 2-29 wherein the detergent is more hydrophillic than lipophilic.

31. A process for the preparation of a pesticidal composition according to any of claims 1-30, the process comprising:

i) dispersing the insecticide phase optionally comprising a detergent and/or a disintegrant in the fatty phase which op-tionally comprises a detergent and/or a disintegrant, provided that at least one of the phases comprises at least one disintegrant, or provided that at least one disintegrant, is added during or after dispersing the insecticide phase in the fatty phase;
ii) subjecting the dispersion of i) to a treatment for obtaining a granular or a powder composition, the method optionally comprising
iii) entrapping air into any of the fatty and insecticide phases and/or into any of the compositions obtained by i) and ii) •

32. The process according to claim 31 wherein the lipid or mixture of lipids is molten during step i) and/or step ii) .

33. The process according to claim 32 wherein the dispersion obtained by step i) is in a molten state and wherein the treatment of ii) involves spray- congealing.

34. A process according to any of claims 31-33 wherein the insecticide phase and/or the fatty phase comprises a detergent .

35. A process according to claim 34 wherein the insecticide phase comprises a nonionic detergent and the fatty phase comprises an anionic detergent.

36. A process according to any of claims 31-35 wherein the pesticidal component is intact Bacillus sphericus and the detergent comprises an cationic detergent.

37. A process according to any of claims 31-36 wherein a filler is added at any step of the process.

38. A process according to any of claims 31-37 wherein an antioxidant and/or a UV protecting agent is added at any step.

39. A process for controlling or combatting larvae of the insects of the order diptera in an aqueous environment comprising applying a composition according to any of claims 1-30 to an aqueous environment harbouring said larvae.

40. A process according to claim 39 wherein the composition applied to the aqueous environment is designed to disinte- grate in an aqueous environment in a substantially gradual rate during a period of from about 3 minutes to more than seven days after application.

41. A process according to claim 39 wherein the composition applied to the aqueous environment is designed to disintegrate in an aqueous environment in a substantially gradual rate during a period of from about 3 minutes to about 50 hours after application.

42. A process according to any of claims 39-41 wherein the disintegrated particles start to sink towards the bottom whereafter the particles reappear at the surface due to the substantial graduate degradation.

43. A process according to claim 42 wherein the disintegrated reappearing particles during the next about 24 hours to about at least seven days continuously travel up and down between the bottom and the surface of the aqueous environment or at any distance therebetween due to further graduate degradation.