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1. (WO1997016528) A MUTANT E. COLI STRAIN WITH INCREASED SUCCINIC ACID PRODUCTION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/1997/016528 International Application No.: PCT/US1996/017661
Publication Date: 09.05.1997 International Filing Date: 31.10.1996
Chapter 2 Demand Filed: 30.05.1997
IPC:
C12N 9/04 (2006.01) ,C12P 7/40 (2006.01) ,C12P 7/44 (2006.01) ,C12P 7/46 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
04
acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
40
containing a carboxyl group
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
40
containing a carboxyl group
44
Polycarboxylic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
40
containing a carboxyl group
44
Polycarboxylic acids
46
Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
Applicants:
THE UNIVERSITY OF CHICAGO [US/US]; 5801 S. Ellis Chicago, IL 60637, US
Inventors:
DONNELLY, Mark; US
MILLARD, Cynthia, S.; US
STOLS, Lucy; US
Agent:
CHERSKOV, Michael, J.; Cherskov & Flaynik The Civic Opera Building Suite 1447 20 North Wacker Drive Chicago, IL 60606, US
Priority Data:
08/556,80502.11.1995US
Title (EN) A MUTANT E. COLI STRAIN WITH INCREASED SUCCINIC ACID PRODUCTION
(FR) SOUCHE DE E. COLI MUTANTE PRODUISANT UNE QUANTITE ACCRUE D'ACIDE SUCCINIQUE
Abstract:
(EN) A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability of catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the $i(E.coli) Group of Bacteria.
(FR) La présente invention concerne un procédé d'isolement de bactéries produisant de l'acide succinique. Ce procédé consiste à accroître la biomasse d'un organisme dépourvu de l'aptitude à cataboliser le pyruvate puis à exposer à une atmosphère anaérobie la biomasse placée dans un milieu de culture riche en glucose de façon à permettre la croissance de mutants catabolisant le pyruvate. L'invention concerne également un mutant, produisant de grandes quantités d'acide succinique, dérivé d'un parent dépourvu des gènes de pyruvate formate lyase et de lactate déshydrogénase, et appartenant au groupe des bactéries $i(E. coli).
Designated States: AL, AM, AT, AU, AZ, BB, BG, BR, BY, CA, CH, CN, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IS, JP, KE, KG, KP, KR, KZ, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, UZ, VN
African Regional Intellectual Property Organization (ARIPO) (KE, LS, MW, SD, SZ, UG)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP0953037US5869301JP2000500333 CN1202930CA2236472KR1019990067095
AU1996076058