Search International and National Patent Collections
Some content of this application is unavailable at the moment.
If this situation persists, please contact us atFeedback&Contact
1. (WO1997015680) CATIONIC POLYACRYLATES AND POLY(ALKYL) ACRYLATES OR THE CORRESPONDING ACRYLAMIDES FOR USE IN SYNTHETIC TRANSFECTION OR BLOCKING SYSTEMS
Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

CLAIMS

1. A synthetic transfection or blocking system comprising as a carrier a cationic, water soluble or water dispersible polyacrylate or poly (Cι_6 alkyl ) acrylate or the corresponding acrylamides .
2. The system according to claim 1, further comprising a plasmid, a gene construct or an oligonucleotide.
3. The system of claim 1 or 2 , wherein a homo or
copolymer of dimethyl amino ethyl methacrylate is the
carrier.
4. The system of any one of claims 1-3, wherein condensed particles comprising the carrier polymer and DNA fragments are enclosed in drug delivery systems such as liposomes or hydrogels .
5. The system of any one of the preceding claims, wherein the carrier polymer is coupled to a homing device.
6. The system of claim 5, wherein the homing device is a monoclonal antibody which is able to recognize a protein present in the cellular membrane of the target cell.
7. The system of any one of the preceding claims, wherein the carrier polymer is coupled to a fusogenic structure.
8. A method for the preparation of a synthetic
transfection or blocking system comprising contacting
polynucleotide fragments with a cationic water soluble or water dispersible polyacrylate or poly(Cι_6 alkyl) acrylate, or the corresponding acrylamides as defined in any one of claims 1-7 under conditions wherein the polymer is positively charged .
9. The method of claim 8, wherein the contacting is carried out in the presence of a viscosity increasing
substance, preferably sucrose.
10. A method for introducing DNA fragments in target cells, comprising contacting these DNA fragments with a polyacrylate, a polyacrylamide, a poly(Cι_6 alkyl) acrylate or a poly (Cι-6 alkyl) acylamide, which is at least partially substituted with cationic substituents and subsequently contacting the obtained transfection system with target cells .
11. Use of a polyacrylate, a polyacrylamide, a poly(Cι_6 alkyl) crylate or a poly(Cι-6 alkyl) acrylamide, which is at least partially substituted with cationic substituents as a DNA carrier system.