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1. WO1997015660 - CRYSTALLINE CELLULASE AND METHOD FOR PRODUCING SAME

Publication Number WO/1997/015660
Publication Date 01.05.1997
International Application No. PCT/US1996/016918
International Filing Date 22.10.1996
Chapter 2 Demand Filed 30.04.1997
IPC
C12N 9/42 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
24acting on glycosyl compounds (3.2)
42acting on beta-1, 4-glucosidic bonds, e.g. cellulase
CPC
C12N 9/2437
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
24acting on glycosyl compounds (3.2)
2402hydrolysing O- and S- glycosyl compounds (3.2.1)
2405Glucanases
2434acting on beta-1,4-glucosidic bonds
2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
C12Y 302/01004
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
302Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
Applicants
  • GENENCOR INTERNATIONAL, INC. [US]/[US]
Inventors
  • BECKER, Nathaniel, T.
  • BRAUNSTEIN, Edit, L.
  • GROS, Ernst, H.
  • FEWKES, Robert
  • HENG, Meng, H.
Agents
  • ANDERSON, Kirsten, A.
Priority Data
547,09623.10.1995US
609,36201.03.1996US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CRYSTALLINE CELLULASE AND METHOD FOR PRODUCING SAME
(FR) CELLULASE CRISTALLINE ET PROCEDE DE PREPARATION ASSOCIE
Abstract
(EN)
A method for preparing a crystalline cellulase enzyme is provided which comprises preparing an aqueous solution containing cellulase enzyme and adding to the aqueous solution a salt comprising an anion selected from the group consisting of sulfate, phosphate, formate, acetate, sorbate, chloride, bromide, fluoride or iodide, and a cation selected from the group consisting of sodium, ammonium, magnesium, potassium or calcium or a mixture thereof.
(FR)
Cette invention concerne un procédé de préparation de cellulase cristalline qui consiste à préparer une solution aqueuse contenant l'enzyme cellulase et à ajouter à cette solution aqueuse un sel contenant un anion sélectionné dans le groupe constitué par un sulfate, un phosphate, un formate, un acétate, un sorbate, un chlorure, un bromure, un fluorure ou un iodure et un cation sélectionné dans le groupe constitué par le sodium, l'ammonium, le magnésium, le potassium, le calcium ou un mélange de ces derniers.
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